| RRC ID |
57825
|
| Author |
Tang JY, Li LJ, Ou-Yang F, Wang CL, Shu CW, Wu KH, Wang HR, Yen CH, Cheng YB, Chang HW.
|
| Title |
Ethyl Acetate Extract of Nepenthes ventricosa x maxima Exerts Preferential Killing to Oral Cancer Cells.
|
| Journal |
DNA Cell Biol
|
| Abstract |
Nepenthes plants are a folk medicine in many Southeast Asia countries for curing diseases but its anticancer effect is rarely investigated. The objectives of this study were to investigate the antioral cancer ability of ethyl acetate extract of Nepenthes ventricosa x maxima (EANV). The preferential killing ability of EANV was determined by MTS-based cell viability assays. The bioactive effects were further screened by flow cytometry for apoptosis, oxidative stress, and DNA damage. At 24 h treatment, EANV dose dependently decreased six types of oral cancer cells, but the normal oral cells (HGF-1) kept a 90% viability. EANV also showed chronic antiproliferative effects and inhibited 3D sphere formation ability of oral cancer cells. Ca9-22 and CAL 27 oral cancer cells with high response to EANV increased subG1 populations and enhanced Annexin V- and pancaspase-detected apoptosis in these cells. EANV also induced the generation of reactive oxygen species (ROS) and mitochondrial superoxide and the dysfunction of mitochondrial membrane potential. Moreover, the oxidative DNA damage level such as 8-oxo-2'deoxyguanosine was increased in EANV-treated oral cancer cells. Taken together, EANV has a preferential killing effect against oral cancer cells associated with oxidative stress, apoptosis, and DNA damage, suggesting EANV as a potential antioral cancer agent.
|
| Volume |
38(8)
|
| Pages |
763-772
|
| Published |
2019-8-1
|
| DOI |
10.1089/dna.2018.4436
|
| PMID |
31170002
|
| MeSH |
Acetates / chemistry
Antineoplastic Agents, Phytogenic / chemistry
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects
Caryophyllales / chemistry*
Cell Line, Tumor
DNA Damage / drug effects
Drug Screening Assays, Antitumor
Humans
Membrane Potential, Mitochondrial / drug effects
Mouth Neoplasms / drug therapy*
Mouth Neoplasms / pathology
Oxidative Stress / drug effects
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Reactive Oxygen Species / metabolism
Spectrometry, Mass, Electrospray Ionization
|
| IF |
3.314
|
| Times Cited |
1
|
| Resource |
| Human and Animal Cells |
Ca9-22(RCB1976)
HSC-3(RCB1975) |
