RRC ID 57853
著者 Maehana S, Matsumoto Y, Kojima F, Kitasato H.
タイトル Interleukin-24 Transduction Modulates Human Prostate Cancer Malignancy Mediated by Regulation of Anchorage Dependence.
ジャーナル Anticancer Res
Abstract BACKGROUND:Hormone therapy and chemotherapy are not effective for castrate-resistant prostate cancer, thus development of novel treatment strategies is required. Gene therapy involving transient high-copy transfection of interleukin (IL)-24 with an adenoviral vector can exert antitumor activity; however, the effects of stable IL-24 transfection are not fully understood. The aim of this study was to investigate the effects of IL-24 overexpression in prostate cancer cells, in vitro.
MATERIALS AND METHODS:DU145 cells were transfected the IL-24 gene using a retroviral vector. Apoptosis induction was investigated by the cell death detection ELISA, and the gene expression was analyzed by real time RT-PCR.
RESULTS:IL-24 transduction suppressed the growth of prostate cancer and induced tumor cell apoptosis. In addition, up-regulation of epithelial markers and down-regulation of mesenchymal markers were noted, suggesting that tumor aggressiveness was reduced.
CONCLUSION:Introduction of IL-24 displays antitumor activity both by induction of apoptosis and regulation of anchorage dependence.
巻・号 39(7)
ページ 3719-3725
公開日 2019-7-1
DOI 10.21873/anticanres.13520
PII 39/7/3719
PMID 31262898
MeSH Apoptosis Cell Proliferation Humans Interleukins / genetics* Male Prostatic Neoplasms / genetics* Prostatic Neoplasms / pathology* Transduction, Genetic
IF 1.994
引用数 0
リソース情報
ヒト・動物細胞 DU145(RCB2143)