RRC ID 57854
Author Mizutani H, Hayashi Y, Hashimoto M, Imai M, Ichimaru Y, Kitamura Y, Ikemura K, Miyazawa D, Ohta K, Ikeda Y, Maeda T, Yoshikawa M, Hiraku Y, Kawanishi S.
Title Oxidative DNA Damage and Apoptosis Induced by Aclarubicin, an Anthracycline: Role of Hydrogen Peroxide and Copper.
Journal Anticancer Res
Abstract BACKGROUND/AIM:This study aimed to investigate aclarubicin (ACR)-induced oxidative DNA damage and apoptosis.
MATERIALS AND METHODS:ACR-induced apoptosis was analyzed using HL-60 leukemia cells and HP100 cells, hydrogen peroxide (H2O2)-resistant cells derived from HL-60 cells. ACR-induced DNA damage was analyzed using plasmid DNA.
RESULTS:HL-60 cells were more sensitive to ACR than HP100 cells. In HP100 cells, DNA ladder formation and caspase-3/7 activity induced by ACR were suppressed or delayed in comparison to those in HL-60 cells. ACR-induced DNA damage occurred in the presence of Cu(II), and scavenger experiments showed that the reactive species causing DNA damage appeared to be generated from H2O2 and Cu(I). Moreover, we detected intracellular Cu(I) induced by ACR in HL-60 cells, using CopperGREEN™, a fluorescent probe for detection of Cu(I) ion specifically.
CONCLUSION:ACR-induced DNA damage and apoptosis can be accounted for by the involvement of H2O2 and Cu(I).
Volume 39(7)
Pages 3443-3451
Published 2019-7-1
DOI 10.21873/anticanres.13490
PII 39/7/3443
PMID 31262868
MeSH Aclarubicin / adverse effects* Antibiotics, Antineoplastic / adverse effects* Apoptosis / drug effects* Cell Line, Tumor Copper / pharmacology* DNA Damage* Humans Hydrogen Peroxide / metabolism* Neoplasms / metabolism
IF 1.935
Times Cited 1
Human and Animal Cells HL-60(RCB3683)