論文 - 詳細
| RRC ID | 57861 |
|---|---|
| 著者 | Odashima A, Onodera S, Saito A, Ogihara Y, Ichinohe T, Azuma T. |
| タイトル | Stage-dependent differential gene expression profiles of cranial neural crest-like cells derived from mouse-induced pluripotent stem cells. |
| ジャーナル | Med Mol Morphol |
| Abstract |
Cranial neural crest cells are multipotent cells that migrate into the pharyngeal arches of the vertebrate embryo and differentiate into various craniofacial organ derivatives. Therefore, migrating cranial neural crest cells are considered one of the most attractive candidate cell sources in regenerative medicine. We generated cranial neural crest like cell (cNCCs) using mouse-induced pluripotent stem cells cultured in neural crest-inducing medium for 14 days. Subsequently, we conducted RNA sequencing experiments to analyze gene expression profiles of cNCCs at different time points after induction. cNCCs expressed several neural crest specifier genes; however, some previously reported specifier genes such as paired box 3 and Forkhead box D3, which are essential for embryonic neural crest development, were not expressed. Moreover, ETS proto-oncogene 1, transcription factor and sex-determining region Y-box 10 were only expressed after 14 days of induction. Finally, cNCCs expressed multiple protocadherins and a disintegrin and metalloproteinase with thrombospondin motifs enzymes, which may be crucial for their migration. |
| 巻・号 | 53(1) |
| ページ | 28-41 |
| 公開日 | 2020-3-1 |
| DOI | 10.1007/s00795-019-00229-2 |
| PII | 10.1007/s00795-019-00229-2 |
| PMID | 31297611 |
| PMC | PMC7033077 |
| MeSH | ADAMTS Proteins / genetics ADAMTS Proteins / metabolism Animals Biomarkers / metabolism Cadherins / genetics Cadherins / metabolism Cell Differentiation Cell Movement DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Embryo, Mammalian Forkhead Transcription Factors / genetics Forkhead Transcription Factors / metabolism Gene Expression Profiling Gene Expression Regulation, Developmental* Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Mice Multipotent Stem Cells / cytology Multipotent Stem Cells / metabolism* Neural Crest / cytology Neural Crest / metabolism* PAX3 Transcription Factor / genetics PAX3 Transcription Factor / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism Sex-Determining Region Y Protein / genetics Sex-Determining Region Y Protein / metabolism Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 2.429 |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | iPS-MEF-Ng-20D-17(APS0001) |