論文 - 詳細
| RRC ID | 57862 |
|---|---|
| 著者 | Kawashima H, Toyooka N, Okada T, Nguyen HD, Nishikawa Y, Miura Y, Inoue N, Kimura KI. |
| タイトル | Identification and Total Synthesis of Two Previously Unreported Odd-Chain Bis-Methylene-Interrupted Fatty Acids with a Terminal Olefin that Activate Protein Phosphatase, Mg2+/Mn2+-Dependent 1A (PPM1A) in Ovaries of the Limpet Cellana toreuma. |
| ジャーナル | Mar Drugs |
| Abstract |
Diverse non-methylene-interrupted (NMI) fatty acids (FAs) with odd-chain lengths have been recognized in triacylglycerols and polar lipids from the ovaries of the limpet Cellana toreuma, however their biological properties remain unclear. In this study, two previously unreported odd-chain NMI FAs, (12Z)-12,16-heptadecadienoic (1) and (14Z)-14,18-nonadecadienoic (2) acids, from the ovary lipids of C. toreuma were identified by a combination of equivalent chain length (ECL) values of their methyl esters and capillary gas chromatography-mass spectrometry (GC-MS) of their 3-pyridylcarbinol derivatives. On the basis of the experimental results, both 1 and 2 were synthesized to prove their structural assignments and to test their biological activity. The ECL values and electron impact-mass (EI-MS) spectra of naturally occurring 1 and 2 were in agreement with those of the synthesized 1 and 2. In an in vitro assay, both 1 and 2 activated protein phosphatase, Mg2+/Mn2+-dependent 1A (PPM1A) up to 100 μM in a dose-dependent manner. |
| 巻・号 | 17(7) |
| 公開日 | 2019-7-11 |
| DOI | 10.3390/md17070410 |
| PII | md17070410 |
| PMID | 31336763 |
| PMC | PMC6669709 |
| MeSH | Animals Biological Products / chemical synthesis Biological Products / pharmacology* Enzyme Activators / chemical synthesis Enzyme Activators / pharmacology* Enzyme Assays Fatty Acids / chemical synthesis Fatty Acids / pharmacology* Female Gastropoda / chemistry* HL-60 Cells Humans Molecular Structure Ovary / metabolism Protein Phosphatase 2C / metabolism* Recombinant Proteins / metabolism |
| IF | 4.073 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |