Reference - Detail
RRC ID | 57884 |
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Author | Goto T, Homma H, Kaida A, Miura M. |
Title | WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells. |
Journal | J Radiat Res |
Abstract |
Hypoxia/reoxygenation (H/R) treatment reportedly induces DNA damage response (DDR), including DNA double-strand break (DSB) repair and G2 arrest, resulting in reduction of clonogenic survival. Because WEE1 plays a key role in the G2/M checkpoint along with CHK1/2, we investigated the effect of WEE1 inhibition on H/R-induced DDR using HeLa cells. The H/R treatment combined with WEE1 inhibitor abrogated G2 arrest, subsequently leading to the cells entering the M phase, and finally resulting in mitotic catastrophe after prolonged mitosis. Colony-forming assay showed an enhanced decrease in the surviving fraction and the focus formation of BRCA1 was significantly reduced. We demonstrate for the first time that WEE1 inhibition enhances H/R-induced cell death accompanied by mitotic catastrophe and that the process may be mediated by homologous recombination. |
Volume | 60(5) |
Pages | 709-713 |
Published | 2019-10-23 |
DOI | 10.1093/jrr/rrz045 |
PII | 5539284 |
PMID | 31347653 |
PMC | PMC6805980 |
MeSH | BRCA1 Protein / metabolism Cell Cycle Proteins / antagonists & inhibitors* Cell Cycle Proteins / metabolism Cell Hypoxia / drug effects Cell Survival / drug effects Clone Cells G2 Phase Cell Cycle Checkpoints / drug effects HeLa Cells Humans Kinetics Mitosis / drug effects Oxygen / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors* Protein-Tyrosine Kinases / metabolism Pyrazoles / pharmacology Pyrimidinones / pharmacology |
IF | 2.014 |
Times Cited | 0 |
Resource | |
Human and Animal Cells | HeLa/Fucci(RCB2812) |