Reference - Detail
| RRC ID | 57884 |
|---|---|
| Author | Goto T, Homma H, Kaida A, Miura M. |
| Title | WEE1 inhibition enhances sensitivity to hypoxia/reoxygenation in HeLa cells. |
| Journal | J Radiat Res |
| Abstract |
Hypoxia/reoxygenation (H/R) treatment reportedly induces DNA damage response (DDR), including DNA double-strand break (DSB) repair and G2 arrest, resulting in reduction of clonogenic survival. Because WEE1 plays a key role in the G2/M checkpoint along with CHK1/2, we investigated the effect of WEE1 inhibition on H/R-induced DDR using HeLa cells. The H/R treatment combined with WEE1 inhibitor abrogated G2 arrest, subsequently leading to the cells entering the M phase, and finally resulting in mitotic catastrophe after prolonged mitosis. Colony-forming assay showed an enhanced decrease in the surviving fraction and the focus formation of BRCA1 was significantly reduced. We demonstrate for the first time that WEE1 inhibition enhances H/R-induced cell death accompanied by mitotic catastrophe and that the process may be mediated by homologous recombination. |
| Volume | 60(5) |
| Pages | 709-713 |
| Published | 2019-10-23 |
| DOI | 10.1093/jrr/rrz045 |
| PII | 5539284 |
| PMID | 31347653 |
| PMC | PMC6805980 |
| MeSH | BRCA1 Protein / metabolism Cell Cycle Proteins / antagonists & inhibitors* Cell Cycle Proteins / metabolism Cell Hypoxia / drug effects Cell Survival / drug effects Clone Cells G2 Phase Cell Cycle Checkpoints / drug effects HeLa Cells Humans Kinetics Mitosis / drug effects Oxygen / pharmacology* Protein-Tyrosine Kinases / antagonists & inhibitors* Protein-Tyrosine Kinases / metabolism Pyrazoles / pharmacology Pyrimidinones / pharmacology |
| IF | 2.014 |
| Times Cited | 0 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa/Fucci(RCB2812) |