論文 - 詳細
| RRC ID | 5789 |
|---|---|
| 著者 | Mizutani M, Naganuma T, Tsutsumi K, Saitoh Y. |
| タイトル | The syncytium-specific expression of the Orysa;KRP3 CDK inhibitor: implication of its involvement in the cell cycle control in the rice (Oryza sativa L.) syncytial endosperm. |
| ジャーナル | J Exp Bot |
| Abstract |
During rice (Oryza sativa L.) seed development, the primary endosperm nucleus undergoes a series of divisions without cytokinesis, producing a multinucleate cell, known as a syncytium. After several rounds of rapid nuclear proliferation, the syncytium ceases to undergo mitosis; thereafter, the syncytium is partitioned into individual cells by a specific type of cytokinesis called cellularization. The transition between syncytium and cellularization is important in determining the final seed size and is a model for studying the cell cycle and cytokinesis. The involvement of cyclin-dependent kinase (CDK) inhibitors (CKIs) in cell cycle control was investigated here during the transition between syncytium and cellularization. It was found that one of the rice CKIs, Orysa;KRP3, is strongly expressed in the caryopsis at 2 d after flowering (DAF), and its expression is significantly reduced at 3 DAF. The other CKI transcripts did not show such a shift at 2 DAF. In situ hybridization analysis revealed that Orysa;KRP3 is expressed in multinucleate syncytial endosperm at 2 DAF, but not in cellularized endosperm at 3 DAF. Two-hybrid assays showed that Orysa;KRP3 binds Orysa;CDKA;1, Orysa;CDKA;2, Orysa;CycA1;1, and Orysa;CycD2;2. By contrast, Orysa;CDKB2;1 and Orysa;CycB2;2 do not show binding to Orysa;KRP3. Orysa;KRP3 was able to rescue yeast premature cell division due to the dominant positive expression of mutant rice CDKA;1 indicating that Orysa;KRP3 inhibited rice CDK. These data suggest that Orysa;KRP3 is involved in cell cycle control of syncytial endosperm. |
| 巻・号 | 61(3) |
| ページ | 791-8 |
| 公開日 | 2010-3-1 |
| DOI | 10.1093/jxb/erp343 |
| PII | erp343 |
| PMID | 19933315 |
| PMC | PMC2814109 |
| MeSH | Cell Cycle / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / metabolism Cyclin-Dependent Kinases / antagonists & inhibitors Cyclins / metabolism Endosperm / cytology* Endosperm / genetics Endosperm / growth & development Flowers / genetics Gene Expression Profiling Gene Expression Regulation, Plant* Genes, Plant / genetics Giant Cells / cytology Giant Cells / metabolism* Organ Specificity / genetics Oryza / anatomy & histology Oryza / cytology Oryza / genetics* Plant Proteins / genetics* Plant Proteins / metabolism Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / cytology |
| IF | 5.908 |
| 引用数 | 33 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY12137 FYP424 |