論文 - 詳細
| RRC ID | 57892 |
|---|---|
| 著者 | Zhang R, Inoue Y, Konno T, Ishihara K. |
| タイトル | Hybridization of a phospholipid polymer hydrogel with a natural extracellular matrix using active cell immobilization. |
| ジャーナル | Biomater Sci |
| Abstract |
Three-dimensional tissue organization is still an obstacle in the field of tissue engineering, which generally involves cell immobilization, proliferation, and organization. As an artificial extracellular matrix (ECM) for providing a suitable environment of cells to construct tissues, combination of cytocompatible polymer hydrogels and natural ECM produced by the immobilized cells was considered. In this research, we designed a spontaneously forming hydrogel system between two water-soluble polymers for the immobilization of cells. These polymers were poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid-co-N-succinimidyloxycarbonyl tetra(ethylene glycol)methacrylate) (PMBVS) and poly(vinyl alcohol) (PVA) to form a PMBVS/PVA hydrogel in a cell culture medium under mild conditions. Basic fibroblast growth factor (bFGF) was conjugated with PMBVS (PMBV-bFGF). To enhance the growth of the immobilized cells, mouse fibroblast L929 cells were immobilized in the PMBVS/PVA hydrogel and the PMBV-bFGF/PVA hydrogel, and their proliferation and secretion of the ECM under stimulation with bFGF was observed. The ECM infiltrated and replaced the hydrogel, resulting in the formation of a hybrid hydrogel with the ECM and laden cells. |
| 巻・号 | 7(7) |
| ページ | 2793-2802 |
| 公開日 | 2019-6-25 |
| DOI | 10.1039/c9bm00093c |
| PMID | 31044192 |
| MeSH | Animals Biocompatible Materials / chemistry Biocompatible Materials / pharmacology* Cell Line Cell Proliferation / drug effects Cells, Immobilized / cytology* Cells, Immobilized / drug effects* Extracellular Matrix / drug effects* Extracellular Matrix / metabolism Fibroblast Growth Factor 2 / chemistry Hydrogels / chemistry* Mice Phospholipids / chemistry* Polymers / chemistry* Polyvinyl Alcohol / chemistry Rheology Solubility Water / chemistry |
| IF | 6.183 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | L929 |