RRC ID 5789
著者 Mizutani M, Naganuma T, Tsutsumi K, Saitoh Y.
タイトル The syncytium-specific expression of the Orysa;KRP3 CDK inhibitor: implication of its involvement in the cell cycle control in the rice (Oryza sativa L.) syncytial endosperm.
ジャーナル J Exp Bot
Abstract During rice (Oryza sativa L.) seed development, the primary endosperm nucleus undergoes a series of divisions without cytokinesis, producing a multinucleate cell, known as a syncytium. After several rounds of rapid nuclear proliferation, the syncytium ceases to undergo mitosis; thereafter, the syncytium is partitioned into individual cells by a specific type of cytokinesis called cellularization. The transition between syncytium and cellularization is important in determining the final seed size and is a model for studying the cell cycle and cytokinesis. The involvement of cyclin-dependent kinase (CDK) inhibitors (CKIs) in cell cycle control was investigated here during the transition between syncytium and cellularization. It was found that one of the rice CKIs, Orysa;KRP3, is strongly expressed in the caryopsis at 2 d after flowering (DAF), and its expression is significantly reduced at 3 DAF. The other CKI transcripts did not show such a shift at 2 DAF. In situ hybridization analysis revealed that Orysa;KRP3 is expressed in multinucleate syncytial endosperm at 2 DAF, but not in cellularized endosperm at 3 DAF. Two-hybrid assays showed that Orysa;KRP3 binds Orysa;CDKA;1, Orysa;CDKA;2, Orysa;CycA1;1, and Orysa;CycD2;2. By contrast, Orysa;CDKB2;1 and Orysa;CycB2;2 do not show binding to Orysa;KRP3. Orysa;KRP3 was able to rescue yeast premature cell division due to the dominant positive expression of mutant rice CDKA;1 indicating that Orysa;KRP3 inhibited rice CDK. These data suggest that Orysa;KRP3 is involved in cell cycle control of syncytial endosperm.
巻・号 61(3)
ページ 791-8
公開日 2010-3-1
DOI 10.1093/jxb/erp343
PII erp343
PMID 19933315
PMC PMC2814109
MeSH Cell Cycle / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / genetics* Cyclin-Dependent Kinase Inhibitor Proteins / metabolism Cyclin-Dependent Kinases / antagonists & inhibitors Cyclins / metabolism Endosperm / cytology* Endosperm / genetics Endosperm / growth & development Flowers / genetics Gene Expression Profiling Gene Expression Regulation, Plant* Genes, Plant / genetics Giant Cells / cytology Giant Cells / metabolism* Organ Specificity / genetics Oryza / anatomy & histology Oryza / cytology Oryza / genetics* Plant Proteins / genetics* Plant Proteins / metabolism Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / cytology
IF 5.908
引用数 33
WOS 分野 PLANT SCIENCES
リソース情報
酵母 FY12137 FYP424