| RRC ID |
57955
|
| 著者 |
Kurashina Y, Imashiro C, Hirano M, Kuribara T, Totani K, Ohnuma K, Friend J, Takemura K.
|
| タイトル |
Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves.
|
| ジャーナル |
Commun Biol
|
| Abstract |
Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. This method detaches 96.2% of the cells, and increases its transfer yield to 130% of conventional methods for 48 h, compared to the number of cells detached by trypsinization. We show the elimination of trypsinization reduces cell damage, improving the survival of the detached cells. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment. This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process.
|
| 巻・号 |
2
|
| ページ |
393
|
| 公開日 |
2019-10-29
|
| DOI |
10.1038/s42003-019-0638-5
|
| PII |
638
|
| PMID |
31701022
|
| PMC |
PMC6820801
|
| MeSH |
Animals
CHO Cells
Cell Adhesion
Cell Proliferation
Cell Separation / instrumentation
Cell Separation / methods*
Cricetulus
Culture Media, Serum-Free
DNA Damage
Equipment Design
Membrane Proteins / metabolism
Microscopy, Electron, Scanning
Trypsin
Ultrasonic Waves
|
| IF |
4.165
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1 (SC)(RCB0403) |
