RRC ID 57991
著者 Umemura Y, Maki I, Tsuchiya Y, Koike N, Yagita K.
タイトル Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells.
ジャーナル J Biol Rhythms
Abstract The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. However, whether a common mechanism regulates the development of the human circadian clock remains unclear. In the present study, we show that human induced pluripotent stem cells (iPSCs) have no discernible circadian molecular oscillation. In addition, in vitro differentiation culture of human iPSCs required a longer duration than that required in mouse for the emergence of circadian oscillations. The expression of CLOCK protein in undifferentiated human iPSCs was posttranscriptionally suppressed despite the expression of CLOCK mRNA, which is consistent with our previous observations in mouse embryonic stem cells, iPSCs, and early mouse embryos. These results suggest that CLOCK protein expressions could be posttranscriptionally suppressed in the early developmental stage not only in mice but also in humans.
巻・号 34(5)
ページ 525-532
公開日 2019-10-1
DOI 10.1177/0748730419865436
PMID 31368392
PMC PMC6732938
MeSH CLOCK Proteins / genetics* CLOCK Proteins / physiology Cell Differentiation* Cells, Cultured Circadian Clocks / genetics* Circadian Clocks / physiology Circadian Rhythm* Gene Expression Regulation Humans Induced Pluripotent Stem Cells / physiology* Protein Processing, Post-Translational* RNA, Messenger / genetics
IF 2.473
引用数 3
リソース情報
ヒト・動物細胞 253G1(HPS0002)