Reference - Detail
| RRC ID | 57994 |
|---|---|
| Author | Iriguchi S, Kaneko S. |
| Title | In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells. |
| Journal | Methods Mol Biol |
| Abstract |
In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells. |
| Volume | 2048 |
| Pages | 93-106 |
| Published | 2019-1-1 |
| DOI | 10.1007/978-1-4939-9728-2_11 |
| PMID | 31396934 |
| MeSH | Animals CD8-Positive T-Lymphocytes / physiology* Cell Differentiation* Cell Line Cell Separation / instrumentation Cell Separation / methods* Coculture Techniques / instrumentation Coculture Techniques / methods Culture Media / metabolism Cytokines / metabolism Fibroblasts Flow Cytometry / instrumentation Flow Cytometry / methods* Fluorescent Antibody Technique, Direct / instrumentation Fluorescent Antibody Technique, Direct / methods Hematopoietic Stem Cells Humans Induced Pluripotent Stem Cells / physiology* Mesenchymal Stem Cells Mice Primates |
| Times Cited | 0 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) |