論文 - 詳細
| RRC ID | 57999 |
|---|---|
| 著者 | Kawai Y, Kaneko S. |
| タイトル | Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC. |
| ジャーナル | Methods Mol Biol |
| Abstract |
In this chapter, we describe redifferentiation procedures from iPSCs to CD8αβ+ cytotoxic T cells in 10 T1/2 and OP9/DL1 feeder condition. iPSC used here is derived from T-cell clone (T-iPSC), which has lost naïve phenotype and acquired exhaustion/senescence phenotype during cloning process (Note 1). On the other hand, redifferentiated T cells (T-iPSC-Ts) reacquire naïve phenotype (CD45RA+CD45RO-CCR7+CD62L+), which are reportedly critical for in vivo persistence of infused T cells and greatly affect therapeutic efficacy of adoptive immunotherapy. Indeed, T-iPSC-Ts exhibit much superior proliferative capacity while retaining equivalent effector function compared to parental T-cell clones. Here, we demonstrate the methodology to produce naïve-like T-iPSC-Ts, which could be potent cell source for adoptive immunotherapy. |
| 巻・号 | 2048 |
| ページ | 71-75 |
| 公開日 | 2019-1-1 |
| DOI | 10.1007/978-1-4939-9728-2_7 |
| PMID | 31396930 |
| MeSH | Animals Cell Culture Techniques / instrumentation Cell Culture Techniques / methods* Cell Differentiation Cell Line Coculture Techniques / instrumentation Coculture Techniques / methods Humans Immunologic Memory Immunotherapy, Adoptive / methods Induced Pluripotent Stem Cells Mesenchymal Stem Cells Mice T-Lymphocytes, Cytotoxic / physiology* T-Lymphocytes, Cytotoxic / transplantation |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) |