RRC ID 580
著者 Taguchi F, Shimizu R, Inagaki Y, Toyoda K, Shiraishi T, Ichinose Y.
タイトル Post-translational modification of flagellin determines the specificity of HR induction.
ジャーナル Plant Cell Physiol
Abstract Flagellin, a constituent of the flagellar filament, is a potent elicitor of hypersensitive cell death in plant cells. Flagellins of Pseudomonas syringae pvs. glycinea and tomato induce hypersensitive cell death in their non-host tobacco plants, whereas those of P. syringae pv. tabaci do not remarkably induce it in its host tobacco plants. However, the deduced amino acid sequences of flagellins from pvs. tabaci and glycinea are identical, indicating that post-translational modification of flagellins plays an important role in determining hypersensitive reaction (HR)-inducibility. To investigate genetically the role of modification of flagellin in HR-induction, biological and phytopathological phenotypes of a flagella-defective Delta fliC mutant and Delta fliC mutants complemented by the introduction of the flagellin gene (fliC) from different pathovars of P. syringae were investigated. The Delta fliC mutant of pv. tabaci lost flagella, motility, the ability to induce HR cell death in non-host tomato cells and virulence toward host tobacco plants, whereas all pv. tabaci complemented by the introduction of the fliC gene of pvs. tabaci, glycinea or tomato recovered all the abilities that the Delta fliC mutant had lost. These results indicate that post-translational modification of flagellins is strongly correlated with the ability to cause HR cell death.
巻・号 44(3)
ページ 342-9
公開日 2003-3-1
DOI 10.1093/pcp/pcg042
PMID 12668781
MeSH Apoptosis Flagellin / genetics* Flagellin / metabolism Genetic Complementation Test Immunoblotting Lycopersicon esculentum / growth & development Lycopersicon esculentum / microbiology Mutation Plant Diseases / microbiology Plant Leaves / growth & development Plant Leaves / microbiology Protein Processing, Post-Translational* Pseudomonas / genetics Pseudomonas / pathogenicity Virulence / genetics
IF 4.062
引用数 66
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00012