論文 - 詳細
| RRC ID | 58037 |
|---|---|
| 著者 | Tachibana K, Kondoh M. |
| タイトル | A Method to Prepare Claudin-Modulating Recombinant Proteins. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The epithelium forms tight junctions by sealing the paracellular space, and tight junctions prevent the free movement of solutes. Claudin is an important structural and functional component of tight junctions and contributes to the formation of paracellular pathways for different populations of size- and charge-selective solutes. Therefore, modulation of tight junctions is important to develop drug delivery strategies. Clostridium perfringens enterotoxin (CPE) causes food poisoning in humans and is a 35-kDa polypeptide, consisting of 319 amino acids and two functional regions. The C-terminal region of CPE (C-CPE) is not cytotoxic and binds to its receptor claudin, which in turn modulates the epithelial tight junction barrier. Thus, claudin binders, such as C-CPE, are useful tools for drug delivery targeting tight junctions. Here, we provide a protocol for the expression and purification of recombinant C-CPE proteins as claudin binders, an analysis method for C-CPE binding affinity, and a procedure for assessing the effect of modulating tight junction integrity. |
| 巻・号 | 2109 |
| ページ | 251-260 |
| 公開日 | 2020-1-1 |
| DOI | 10.1007/7651_2019_258 |
| PMID | 31471875 |
| MeSH | Caco-2 Cells Claudins / genetics* Claudins / metabolism Clostridium perfringens / chemistry Clostridium perfringens / metabolism* Electric Impedance Enterotoxins / chemistry Enterotoxins / genetics* Enterotoxins / metabolism Humans Protein Domains Protein Engineering Recombinant Proteins / metabolism Tight Junctions / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |