論文 - 詳細
| RRC ID | 58054 |
|---|---|
| 著者 | Sugawara Y, Hamada K, Yamada Y, Kumai J, Kanagawa M, Kobayashi K, Toda T, Negishi Y, Katagiri F, Hozumi K, Nomizu M, Kikkawa Y. |
| タイトル | Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment. |
| ジャーナル | Sci Rep |
| Abstract |
Human induced pluripotent stem cells (hiPSCs) grow indefinitely in culture and have the potential to regenerate various tissues. In the development of cell culture systems, a fragment of laminin-511 (LM511-E8) was found to improve the proliferation of stem cells. The adhesion of undifferentiated cells to LM511-E8 is mainly mediated through integrin α6β1. However, the involvement of non-integrin receptors remains unknown in stem cell culture using LM511-E8. Here, we show that dystroglycan (DG) is strongly expressed in hiPSCs. The fully glycosylated DG is functionally active for laminin binding, and although it has been suggested that LM511-E8 lacks DG binding sites, the fragment does weakly bind to DG. We further identified the DG binding sequence in LM511-E8, using synthetic peptides, of which, hE8A5-20 (human laminin α5 2688-2699: KTLPQLLAKLSI) derived from the laminin coiled-coil domain, exhibited DG binding affinity and cell adhesion activity. Deletion and mutation studies show that LLAKLSI is the active core sequence of hE8A5-20, and that, K2696 is a critical amino acid for DG binding. We further demonstrated that hiPSCs adhere to hE8A5-20-conjugated chitosan matrices. The amino acid sequence of DG binding peptides would be useful to design substrata for culture system of undifferentiated and differentiated stem cells. |
| 巻・号 | 9(1) |
| ページ | 13037 |
| 公開日 | 2019-9-10 |
| DOI | 10.1038/s41598-019-49669-x |
| PII | 10.1038/s41598-019-49669-x |
| PMID | 31506597 |
| PMC | PMC6737067 |
| MeSH | Amino Acid Sequence Biomarkers Cell Adhesion* Cells, Cultured Dystroglycans / metabolism* Extracellular Matrix Fluorescent Antibody Technique Humans Induced Pluripotent Stem Cells / metabolism* Laminin / chemistry Laminin / metabolism* Peptide Fragments / chemistry Peptide Fragments / metabolism* Protein Binding Protein Transport |
| IF | 3.998 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 454E2(HPS0077) |