論文 - 詳細
| RRC ID | 58063 |
|---|---|
| 著者 | Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S. |
| タイトル | Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:γ-Glutamylcyclotransferase (GGCT) is highly expressed in many forms of cancer, and is a promising therapeutic target. The present study investigated whether inhibition of GGCT enhanced the antiproliferative effects of the drug docetaxel in prostate cancer cells. MATERIALS AND METHODS:Immunohistochemistry and western blot analysis were conducted to measure GGCT expression in prostate cancer tissue samples and cell lines. GGCT was inhibited using RNAi and a novel enzymatic inhibitor, pro-GA, and cell proliferation was evaluated with single and combination treatments of GGCT inhibitors and docetaxel. RESULTS:GGCT was highly expressed in cultured prostate cancer cells and patient samples. GGCT inhibition alone inhibited prostate cancer cell line proliferation and induced cellular senescence. GGCT inhibition in combination with apoptosis-inducing docetaxel had more potent antiproliferative effects than either drug used alone. CONCLUSION:GGCT inhibition may potentiate anticancer drug efficacy. |
| 巻・号 | 39(9) |
| ページ | 4811-4816 |
| 公開日 | 2019-9-1 |
| DOI | 10.21873/anticanres.13666 |
| PII | 39/9/4811 |
| PMID | 31519583 |
| MeSH | Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Cell Proliferation / drug effects Cellular Senescence / drug effects Docetaxel / pharmacology* Enzyme Inhibitors / pharmacology* Gene Expression Humans Immunohistochemistry Male Prostatic Neoplasms / drug therapy Prostatic Neoplasms / metabolism Prostatic Neoplasms / pathology RNA, Small Interfering / genetics gamma-Glutamylcyclotransferase / antagonists & inhibitors* gamma-Glutamylcyclotransferase / genetics gamma-Glutamylcyclotransferase / metabolism |
| IF | 1.994 |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-3(RCB2145) HL-60(RCB3683) LNCap.FGC(RCB2144) |