論文 - 詳細
| RRC ID | 58126 |
|---|---|
| 著者 | Yokota S, Kuramochi H, Okubo K, Iwaya A, Tsuchiya S, Ichiki T. |
| タイトル | Extracellular vesicles nanoarray technology: Immobilization of individual extracellular vesicles on nanopatterned polyethylene glycol-lipid conjugate brushes. |
| ジャーナル | PLoS One |
| Abstract |
Arraying individual extracellular vesicles (EVs) on a chip is expected one of the promising approaches for investigating their inherent properties. In this study, we immobilized individual EVs on a surface using a nanopatterned tethering chip-based versatile platform. A microfluidic device was used to ensure soft, reproducible exposure of the EVs over the whole chip surface. The device is incorporated with a high-density nanoarray chip patterned with 200-nm diameter nanospots composed of polyethylene glycol (PEG)-lipid conjugate brushes. We present a procedure adopted for fabricating high-density PEG-lipid modified nanospots (200 nmϕ, 5.0 × 105 spots/mm2 in 2 × 2 mm2 area). This procedure involves nanopatterning using electron beam lithography, followed by multistep selective chemical modification. Aqueous treatment of a silane coupling agent, used as a linker between PEG-lipid molecules and the silicon surface, was the key step that enabled surface modification using a nanopatterned resist film as a mask. The nanoarray chip was removed from the device for subsequent measurements such as atomic force microscopy (AFM). We developed a prototype device and individually immobilized EVs derived from different cell lines (Sk-Br-3 and HEK293) on tethering nanospots. We characterized EV's morphology using AFM and showed the possibility of evaluating the deformability of EVs using the aspect ratio as an indicator. |
| 巻・号 | 14(10) |
| ページ | e0224091 |
| 公開日 | 2019-10-24 |
| DOI | 10.1371/journal.pone.0224091 |
| PII | PONE-D-19-13842 |
| PMID | 31648253 |
| PMC | PMC6812765 |
| MeSH | Extracellular Vesicles / chemistry* Extracellular Vesicles / metabolism* HEK293 Cells Humans Lab-On-A-Chip Devices* Lipids / chemistry* Microscopy, Atomic Force Nanotechnology / instrumentation* Nanotechnology / methods Polyethylene Glycols / chemistry* |
| IF | 2.74 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |