| RRC ID |
58126
|
| 著者 |
Yokota S, Kuramochi H, Okubo K, Iwaya A, Tsuchiya S, Ichiki T.
|
| タイトル |
Extracellular vesicles nanoarray technology: Immobilization of individual extracellular vesicles on nanopatterned polyethylene glycol-lipid conjugate brushes.
|
| ジャーナル |
PLoS One
|
| Abstract |
Arraying individual extracellular vesicles (EVs) on a chip is expected one of the promising approaches for investigating their inherent properties. In this study, we immobilized individual EVs on a surface using a nanopatterned tethering chip-based versatile platform. A microfluidic device was used to ensure soft, reproducible exposure of the EVs over the whole chip surface. The device is incorporated with a high-density nanoarray chip patterned with 200-nm diameter nanospots composed of polyethylene glycol (PEG)-lipid conjugate brushes. We present a procedure adopted for fabricating high-density PEG-lipid modified nanospots (200 nmϕ, 5.0 × 105 spots/mm2 in 2 × 2 mm2 area). This procedure involves nanopatterning using electron beam lithography, followed by multistep selective chemical modification. Aqueous treatment of a silane coupling agent, used as a linker between PEG-lipid molecules and the silicon surface, was the key step that enabled surface modification using a nanopatterned resist film as a mask. The nanoarray chip was removed from the device for subsequent measurements such as atomic force microscopy (AFM). We developed a prototype device and individually immobilized EVs derived from different cell lines (Sk-Br-3 and HEK293) on tethering nanospots. We characterized EV's morphology using AFM and showed the possibility of evaluating the deformability of EVs using the aspect ratio as an indicator.
|
| 巻・号 |
14(10)
|
| ページ |
e0224091
|
| 公開日 |
2019-10-24
|
| DOI |
10.1371/journal.pone.0224091
|
| PII |
PONE-D-19-13842
|
| PMID |
31648253
|
| PMC |
PMC6812765
|
| MeSH |
Extracellular Vesicles / chemistry*
Extracellular Vesicles / metabolism*
HEK293 Cells
Humans
Lab-On-A-Chip Devices*
Lipids / chemistry*
Microscopy, Atomic Force
Nanotechnology / instrumentation*
Nanotechnology / methods
Polyethylene Glycols / chemistry*
|
| IF |
2.74
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
293(RCB1637) |
