Reference - Detail
| RRC ID | 58227 |
|---|---|
| Author | Ogura T, Yokoyama T, Fujisawa H, Kurashima Y, Esumi H. |
| Title | Structural diversity of neuronal nitric oxide synthase mRNA in the nervous system. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Nitric oxide (NO), generated from L-arginine by an enzymatic reaction of a NO synthase (NOS; EC 1.14.23), is a recently identified biological mediator suggested to be involved in a wide variety of biological processes. In the present work, we isolated and sequenced the entire region of cDNAs encoding mouse neuronal NOS (n-NOS) and demonstrated structural diversity of n-NOS mRNA in the nervous system. Sequence determination revealed a novel mRNA with an inframe deletion in the middle of the n-NOS cDNA. Structural analysis of the corresponding part of the n-NOS gene indicated that the deletion corresponded exactly to two exons. These findings suggest that the variant n-NOS is formed by alternative splicing. Both n-NOSs were found in almost all parts of the nervous system, the expression level of the novel variant being about one twentieth of that of the known n-NOS. Generation of diversity of n-NOS is probably related to diversity of its biological functions. |
| Volume | 193(3) |
| Pages | 1014-22 |
| Published | 1993-6-30 |
| DOI | 10.1006/bbrc.1993.1726 |
| PII | S0006-291X(83)71726-2 |
| PMID | 7686743 |
| MeSH | Amino Acid Oxidoreductases / biosynthesis* Amino Acid Oxidoreductases / genetics* Amino Acid Sequence Animals Base Sequence Brain / enzymology* Cloning, Molecular Gene Library Genetic Variation* Mice Mice, Inbred BALB C Molecular Sequence Data Neurons / enzymology* Nitric Oxide Synthase Oligodeoxyribonucleotides Organ Specificity Poly A / genetics Poly A / isolation & purification Polymerase Chain Reaction RNA / genetics RNA / isolation & purification RNA, Messenger / genetics* RNA, Messenger / metabolism* Spinal Cord / enzymology* |
| IF | 2.985 |
| Times Cited | 113 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Mouse Neuronal NOS cDNA (RDB01386) |