| RRC ID |
58227
|
| Author |
Ogura T, Yokoyama T, Fujisawa H, Kurashima Y, Esumi H.
|
| Title |
Structural diversity of neuronal nitric oxide synthase mRNA in the nervous system.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Nitric oxide (NO), generated from L-arginine by an enzymatic reaction of a NO synthase (NOS; EC 1.14.23), is a recently identified biological mediator suggested to be involved in a wide variety of biological processes. In the present work, we isolated and sequenced the entire region of cDNAs encoding mouse neuronal NOS (n-NOS) and demonstrated structural diversity of n-NOS mRNA in the nervous system. Sequence determination revealed a novel mRNA with an inframe deletion in the middle of the n-NOS cDNA. Structural analysis of the corresponding part of the n-NOS gene indicated that the deletion corresponded exactly to two exons. These findings suggest that the variant n-NOS is formed by alternative splicing. Both n-NOSs were found in almost all parts of the nervous system, the expression level of the novel variant being about one twentieth of that of the known n-NOS. Generation of diversity of n-NOS is probably related to diversity of its biological functions.
|
| Volume |
193(3)
|
| Pages |
1014-22
|
| Published |
1993-6-30
|
| DOI |
10.1006/bbrc.1993.1726
|
| PII |
S0006-291X(83)71726-2
|
| PMID |
7686743
|
| MeSH |
Amino Acid Oxidoreductases / biosynthesis*
Amino Acid Oxidoreductases / genetics*
Amino Acid Sequence
Animals
Base Sequence
Brain / enzymology*
Cloning, Molecular
Gene Library
Genetic Variation*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Neurons / enzymology*
Nitric Oxide Synthase
Oligodeoxyribonucleotides
Organ Specificity
Poly A / genetics
Poly A / isolation & purification
Polymerase Chain Reaction
RNA / genetics
RNA / isolation & purification
RNA, Messenger / genetics*
RNA, Messenger / metabolism*
Spinal Cord / enzymology*
|
| IF |
2.985
|
| Times Cited |
113
|
| Resource |
| DNA material |
Mouse Neuronal NOS cDNA (RDB01386) |
