| RRC ID |
58237
|
| 著者 |
Nakamura S, Hira S, Fujiwara M, Miyagata N, Tsuji T, Kondo A, Kimura H, Shinozuka Y, Hayashi M, Kobayashi S, Mukai M.
|
| タイトル |
A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos.
|
| ジャーナル |
Commun Biol
|
| Abstract |
Expression of the vasa gene is associated with germline establishment. Therefore, identification of vasa activator(s) should provide insights into germline development. However, the genes sufficient for vasa activation remain unknown. Previously, we showed that the BTB/POZ-Zn-finger protein Mamo is necessary for vasa expression in Drosophila. Here, we show that the truncated Mamo lacking the BTB/POZ domain (MamoAF) is a potent vasa activator. Overexpression of MamoAF was sufficient to induce vasa expression in both primordial germ cells and brain. Indeed, Mamo mRNA encoding a truncated Mamo isoform, which is similar to MamoAF, was predominantly expressed in primordial germ cells. The results of our genetic and biochemical studies showed that MamoAF, together with CBP, epigenetically activates vasa expression. Furthermore, MamoAF and the germline transcriptional activator OvoB exhibited synergy in activating vasa transcription. We propose that a Mamo-mediated network of epigenetic and transcriptional regulators activates vasa expression.
|
| 巻・号 |
2
|
| ページ |
422
|
| 公開日 |
2019-11-20
|
| DOI |
10.1038/s42003-019-0663-4
|
| PII |
663
|
| PMID |
31799425
|
| PMC |
PMC6868150
|
| MeSH |
Animals
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Drosophila / embryology*
Drosophila / genetics*
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism
Embryonic Development / genetics*
Epigenesis, Genetic
Gene Expression Regulation, Developmental
Models, Biological
Phenotype
Sequence Deletion*
Transcription Factors / genetics*
Transcription Factors / metabolism
|
| IF |
4.165
|
| リソース情報 |
| ショウジョウバエ |
|
