RRC ID 5825
Author Tamura K, Mawaribuchi S, Yoshimoto S, Shiba T, Takamatsu N, Ito M.
Title Tumor necrosis factor-related apoptosis-inducing ligand 1 (TRAIL1) enhances the transition of red blood cells from the larval to adult type during metamorphosis in Xenopus.
Journal Blood
Abstract The transition of red blood cells (RBCs) from primitive to definitive erythropoiesis is conserved across vertebrates. In anuran amphibians, the larval RBCs from primitive erythropoiesis are replaced by adult RBCs from definitive erythropoiesis during metamorphosis. The molecular mechanisms by which the primitive (larval) blood cells are specifically removed from circulation are not yet understood. In this study, we identified Xenopus tumor necrosis factor-related apoptosis-inducing ligand 1 (xTRAIL1) and xTRAIL2 as ligands of Xenopus death receptor-Ms (xDR-Ms) and investigated whether TRAIL signaling could be involved in this transition. The Trail and xDR-M genes were highly expressed in the liver and RBCs, respectively, during metamorphosis. Interestingly, xTRAIL1 enhanced the transition of the RBCs, and a dominant-negative form of the xTRAIL1 receptor attenuated it, when injected into tadpoles. Moreover, xTRAIL1 induced apoptosis in larval RBCs, but had little effect on adult RBCs in vitro. We also found that adult RBCs treated with staurosporine, a protein kinase C (PKC) inhibitor, were sensitized to xTRAIL1. The mRNAs for PKC isoforms were up-regulated in RBCs during metamorphosis. These results suggest that xTRAIL1 can cause apoptosis, probably mediated through xDR-Ms, in larval RBCs, but may not kill adult RBCs, presumably owing to PKC activation, as part of the mechanism for RBC switching.
Volume 115(4)
Pages 850-9
Published 2010-1-28
DOI 10.1182/blood-2009-04-218966
PII blood-2009-04-218966
PMID 19965624
MeSH Animals Apoptosis / physiology Carrier Proteins / genetics* Carrier Proteins / metabolism* Caspase 3 / metabolism Cells, Cultured Epithelial Cells / cytology Epithelial Cells / physiology Erythrocytes / cytology* Erythrocytes / physiology Kidney / cytology Metamorphosis, Biological / physiology* Molecular Sequence Data Phylogeny Polymerase Chain Reaction Protein Kinase C / genetics Protein Kinase C / metabolism Protein Structure, Tertiary RNA, Messenger / metabolism Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Receptors, TNF-Related Apoptosis-Inducing Ligand / chemistry Receptors, TNF-Related Apoptosis-Inducing Ligand / genetics Receptors, TNF-Related Apoptosis-Inducing Ligand / metabolism Signal Transduction / physiology Transfection Xenopus Proteins / chemistry Xenopus Proteins / genetics* Xenopus Proteins / metabolism* Xenopus laevis / genetics* Xenopus laevis / growth & development
IF 15.132
Times Cited 3
WOS Category HEMATOLOGY
Resource
DNA material pRx-crmA-bsr (RDB01890)