Reference - Detail
| RRC ID | 58260 |
|---|---|
| Author | Tsuji A, Kikuchi Y, Sato Y, Koide S, Yuasa K, Nagahama M, Matsuda Y. |
| Title | A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4. |
| Journal | Biochem J |
| Abstract |
SPCs (subtilisin-like proprotein convertases) are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins. In an effort to examine the substrate protein for PACE4 (paired basic amino-acid-cleaving enzyme-4), an SPC, a potent protein inhibitor of PACE4, an alpha1-antitrypsin RVRR (Arg-Val-Arg-Arg) variant, was expressed in GH4C1 cells. Ectopic expression of the RVRR variant caused accumulation of the 48 kDa protein in cells. Sequence analysis indicates that the 48 kDa protein is a putative Ca2+-binding protein, RCN-3 (reticulocalbin-3), which had previously been predicted by bioinformatic analysis of cDNA from the human hypothalamus. RCN-3 is a member of the CREC (Cab45/reticulocalbin/ERC45/calumenin) family of multiple EF-hand Ca2+-binding proteins localized to the secretory pathway. The most interesting feature of the RCN-3 sequence is the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of SPCs. Biosynthetic studies showed that RCN-3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca2+ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells. Furthermore, autoactivation and secretion of PACE4 was increased upon co-expression with RCN-3. Our findings suggest that selective and transient association of RCN-3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4. |
| Volume | 396(1) |
| Pages | 51-9 |
| Published | 2006-5-15 |
| DOI | 10.1042/BJ20051524 |
| PII | BJ20051524 |
| PMID | 16433634 |
| PMC | PMC1449992 |
| MeSH | Adenoma / pathology Amino Acid Sequence Animals COS Cells Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Cell Line Cell Line, Tumor / metabolism Chlorocebus aethiops Enzyme Activation Furin / antagonists & inhibitors Humans Kidney / cytology Molecular Sequence Data Neoplasm Proteins / metabolism Pituitary Neoplasms / pathology Proprotein Convertase 5 / antagonists & inhibitors Proprotein Convertases Protein Binding Protein Precursors / genetics Protein Precursors / metabolism* Proteomics Rats Recombinant Fusion Proteins / metabolism Sequence Alignment Sequence Homology, Amino Acid Serine Endopeptidases / genetics Serine Endopeptidases / metabolism* Transfection alpha 1-Antitrypsin / chemistry alpha 1-Antitrypsin / genetics alpha 1-Antitrypsin / metabolism |
| IF | 4.097 |
| Times Cited | 24 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Human Reticulocalbin 3 (RDB03412) |