論文 - 詳細
| RRC ID | 58336 |
|---|---|
| 著者 | Girão H, Okada N, Rodrigues TA, Silva AO, Figueiredo AC, Garcia Z, Moutinho-Santos T, Hayashi I, Azevedo JE, Macedo-Ribeiro S, Maiato H. |
| タイトル | CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments. |
| ジャーナル | J Cell Biol |
| Abstract |
CLASPs are conserved microtubule plus-end-tracking proteins that suppress microtubule catastrophes and independently localize to kinetochores during mitosis. Thus, CLASPs are ideally positioned to regulate kinetochore-microtubule dynamics required for chromosome segregation fidelity, but the underlying mechanism remains unknown. Here, we found that human CLASP2 exists predominantly as a monomer in solution, but it can self-associate through its C-terminal kinetochore-binding domain. Kinetochore localization was independent of self-association, and driving monomeric CLASP2 to kinetochores fully rescued normal kinetochore-microtubule dynamics, while partially sustaining mitosis. CLASP2 kinetochore localization, recognition of growing microtubule plus-ends through EB-protein interaction, and the ability to associate with curved microtubule protofilaments through TOG2 and TOG3 domains independently sustained normal spindle length, timely spindle assembly checkpoint satisfaction, chromosome congression, and faithful segregation. Measurements of kinetochore-microtubule half-life and poleward flux revealed that CLASP2 regulates kinetochore-microtubule dynamics by integrating distinctive microtubule-binding properties at the kinetochore-microtubule interface. We propose that kinetochore CLASP2 suppresses microtubule depolymerization and detachment by binding to curved protofilaments at microtubule plus-ends. |
| 巻・号 | 219(2) |
| 公開日 | 2020-2-3 |
| DOI | 10.1083/jcb.201905080 |
| PII | jcb.201905080 |
| PMID | 31757788 |
| PMC | PMC7041679 |
| MeSH | Chromosome Segregation / genetics HeLa Cells Humans Kinetochores / metabolism* M Phase Cell Cycle Checkpoints / genetics* Microtubule-Associated Proteins / genetics* Microtubules / genetics Mitosis / genetics Protein Binding / genetics Protein Domains Spindle Apparatus / genetics |
| IF | 8.811 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 35 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS (RDB04377) |