論文 - 詳細
| RRC ID | 58363 |
|---|---|
| 著者 | Yamano T, Fukuzawa H. |
| タイトル | Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions. |
| 巻・号 | 2050 |
| ページ | 155-161 |
| 公開日 | 2020-1-1 |
| DOI | 10.1007/978-1-4939-9740-4_16 |
| PMID | 31468489 |
| MeSH | Cell Wall Chlamydomonas reinhardtii / genetics* Chlamydomonas reinhardtii / growth & development Electroporation / instrumentation* Equipment Design Transfection / instrumentation* Transformation, Genetic |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-2235 |