RRC ID 58369
Author Fujii S, Tajiri Y, Hasegawa K, Matsumoto S, Yoshimoto RU, Wada H, Kishida S, Kido MA, Yoshikawa H, Ozeki S, Kiyoshima T.
Title The TRPV4-AKT axis promotes oral squamous cell carcinoma cell proliferation via CaMKII activation.
Journal Lab Invest
Abstract Most human malignant tumor cells arise from epithelial tissues, which show distinctive characteristics, such as polarization, cell-to-cell contact between neighboring cells, and anchoring to a basement membrane. When tumor cells invaginate into the stroma, the cells are exposed to extracellular environments, including the extracellular matrix (ECM). Increased ECM stiffness has been reported to promote cellular biological activities, such as excessive cellular growth and enhanced migration capability. Therefore, tumorous ECM stiffness is not only an important clinical tumor feature but also plays a pivotal role in tumor cell behavior. Transient receptor potential vanilloid 4 (TRPV4), a Ca2+-permeable nonselective cation channel, has been reported to be mechano-sensitive and to regulate tumorigenesis, but the underlying molecular mechanism in tumorigenesis remains unclear. The function of TRPV4 in oral squamous cell carcinoma (OSCC) is also unknown. The current study was conducted to investigate whether or not TRPV4 might be involved in OSCC tumorigenesis. TRPV4 mRNA levels were elevated in OSCC cell lines compared with normal oral epithelial cells, and its expression was required for TRPV4 agonist-dependent Ca2+ entry. TRPV4-depleted tumor cells exhibited decreased proliferation capabilities in three-dimensional culture but not in a low-attachment plastic dish. A xenograft tumor model demonstrated that TRPV4 expression was involved in cancer cell proliferation in vivo. Furthermore, loss-of-function experiments using siRNA or an inhibitor revealed that the TRPV4 expression was required for CaMKII-mediated AKT activation. Immunohistochemical analyses of tissue specimens obtained from 36 OSCC patients showed that TRPV4 was weakly observed in non-tumor regions but was strongly expressed in tumor lesions at high frequencies where phosphorylated AKT expression was frequently detected. These results suggest that the TRPV4/CaMKII/AKT axis, which might be activated by extracellular environments, promotes OSCC tumor cell growth.
Volume 100(2)
Pages 311-323
Published 2020-2-1
DOI 10.1038/s41374-019-0357-z
PII 10.1038/s41374-019-0357-z
PMID 31857698
MeSH Animals Calcium-Calmodulin-Dependent Protein Kinase Type 2 / metabolism* Carcinoma, Squamous Cell* / metabolism Carcinoma, Squamous Cell* / physiopathology Cell Line, Tumor Cell Movement / physiology Cell Proliferation / physiology* Cells, Cultured Gene Knockdown Techniques Humans Male Mice Mice, Inbred BALB C Mouth Neoplasms* / metabolism Mouth Neoplasms* / physiopathology Proto-Oncogene Proteins c-akt / metabolism Signal Transduction TRPV Cation Channels* / genetics TRPV Cation Channels* / metabolism
IF 3.684
Times Cited 0
Resource
DNA material CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393)
Human and Animal Cells SAS(RCB1974) HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902)