| RRC ID |
5838
|
| Author |
Herpin A, Nakamura S, Wagner TU, Tanaka M, Schartl M.
|
| Title |
A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Differential gene expression largely accounts for the coordinated manifestation of the genetic programme underlying embryonic development and cell differentiation. The 3' untranslated region (3'-UTR) of eukaryotic genes can contain motifs involved in regulation of gene expression at the post-transcriptional level. In the 3'-UTR of dmrt1, a key gene that functions in gonad development and differentiation, an 11-bp protein-binding motif was identified that mediates gonad-specific mRNA localization during embryonic and larval development of fish. Mutations that disrupt the 11-bp motif leading to in vitro protein-binding loss and selective transcript stabilization failure indicate a role for this motif in RNA stabilization through protein binding. The sequence motif was found to be conserved in most of the dmrt1 homologous genes from flies to humans suggesting a widespread conservation of this specific mechanism.
|
| Volume |
37(5)
|
| Pages |
1510-20
|
| Published |
2009-4-1
|
| DOI |
10.1093/nar/gkn1065
|
| PII |
gkn1065
|
| PMID |
19139075
|
| PMC |
PMC2655695
|
| MeSH |
3' Untranslated Regions / chemistry*
Animals
Cells, Cultured
Fish Proteins / biosynthesis
Fish Proteins / genetics*
Gene Expression Regulation, Developmental*
Gonads / embryology
Gonads / growth & development
Gonads / metabolism*
Humans
Mesoderm / metabolism
Oryzias / embryology
Oryzias / genetics
Oryzias / growth & development
Protein Biosynthesis
RNA Stability
RNA, Messenger / metabolism
RNA-Binding Proteins / metabolism
Regulatory Sequences, Ribonucleic Acid*
Transcription Factors / biosynthesis
Transcription Factors / genetics*
|
| IF |
11.502
|
| Times Cited |
13
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Medaka |
Carbio strain (Cab) |
