論文 - 詳細
| RRC ID | 58392 |
|---|---|
| 著者 | Araki R, Hoki Y, Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M. |
| タイトル | Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency. |
| ジャーナル | Nat Commun |
| Abstract |
A number of point mutations have been identified in reprogrammed pluripotent stem cells such as iPSCs and ntESCs. The molecular basis for these mutations has remained elusive however, which is a considerable impediment to their potential medical application. Here we report a specific stage at which iPSC generation is not reduced in response to ionizing radiation, i.e. radio-resistance. Quite intriguingly, a G1/S cell cycle checkpoint deficiency occurs in a transient fashion at the initial stage of the genome reprogramming process. These cancer-like phenomena, i.e. a cell cycle checkpoint deficiency resulting in the accumulation of point mutations, suggest a common developmental pathway between iPSC generation and tumorigenesis. This notion is supported by the identification of specific cancer mutational signatures in these cells. We describe efficient generation of human integration-free iPSCs using erythroblast cells, which have only a small number of point mutations and INDELs, none of which are in coding regions. |
| 巻・号 | 11(1) |
| ページ | 197 |
| 公開日 | 2020-1-10 |
| DOI | 10.1038/s41467-019-13830-x |
| PII | 10.1038/s41467-019-13830-x |
| PMID | 31924765 |
| PMC | PMC6954237 |
| MeSH | Animals Cell Division Cellular Reprogramming Erythroblasts G1 Phase Cell Cycle Checkpoints / genetics* G1 Phase Cell Cycle Checkpoints / radiation effects Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism* Induced Pluripotent Stem Cells / radiation effects Neoplasms / genetics Open Reading Frames Point Mutation S Phase Cell Cycle Checkpoints / genetics* S Phase Cell Cycle Checkpoints / radiation effects X-Rays |
| IF | 12.121 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02290 |
| ヒト・動物細胞 | Jurkat(RCB3052) |