RRC ID 58393
Author Imaizumi Y, Furutachi S, Watanabe T, Miya H, Kawaguchi D, Gotoh Y.
Title Role of the imprinted allele of the Cdkn1c gene in mouse neocortical development.
Journal Sci Rep
Abstract Imprinted genes are expressed from only one allele in a parent of origin-specific manner. The cyclin-dependent kinase inhibitor p57kip2 is encoded by an imprinted gene Cdkn1c, with the paternal allele being silenced. The possible expression and function of the paternal allele of Cdkn1c have remained little studied, however. We now show that the paternal allele of the Cdkn1c gene is expressed at a low level in the developing mouse neocortex. Surprisingly, the central nervous system-specific conditional deletion of the paternal allele (pat cKO) at the Cdkn1c locus resulted in a marked reduction in brain size. Furthermore, pat cKO gradually reduced the number of neural stem-progenitor cells (NPCs) during neocortical development, and thus reduced the number of upper-layer neurons, which were derived from late-stage NPCs. Our results thus show that the paternal allele of the Cdkn1c locus plays a key role in maintenance of NPCs during neocortical development.
Volume 10(1)
Pages 1884
Published 2020-2-5
DOI 10.1038/s41598-020-58629-9
PII 10.1038/s41598-020-58629-9
PMID 32024956
PMC PMC7002495
MeSH Alleles Animals Cell Differentiation / genetics Cyclin-Dependent Kinase Inhibitor p57 / genetics* Cyclin-Dependent Kinase Inhibitor p57 / metabolism Embryo, Mammalian Embryonic Development / genetics* Female Gene Expression Profiling Genomic Imprinting* Male Mice Mice, Knockout Neocortex / cytology Neocortex / embryology* Neural Stem Cells / physiology* Neurons / physiology
IF 3.998
Times Cited 1
Mice RBRC00639