論文 - 詳細
| RRC ID | 58415 |
|---|---|
| 著者 | Waki T, Mameda R, Nakano T, Yamada S, Terashita M, Ito K, Tenma N, Li Y, Fujino N, Uno K, Yamashita S, Aoki Y, Denessiouk K, Kawai Y, Sugawara S, Saito K, Yonekura-Sakakibara K, Morita Y, Hoshino A, Takahashi S, Nakayama T. |
| タイトル | A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity. |
| ジャーナル | Nat Commun |
| Abstract |
Land plants produce diverse flavonoids for growth, survival, and reproduction. Chalcone synthase is the first committed enzyme of the flavonoid biosynthetic pathway and catalyzes the production of 2',4,4',6'-tetrahydroxychalcone (THC). However, it also produces other polyketides, including p-coumaroyltriacetic acid lactone (CTAL), because of the derailment of the chalcone-producing pathway. This promiscuity of CHS catalysis adversely affects the efficiency of flavonoid biosynthesis, although it is also believed to have led to the evolution of stilbene synthase and p-coumaroyltriacetic acid synthase. In this study, we establish that chalcone isomerase-like proteins (CHILs), which are encoded by genes that are ubiquitous in land plant genomes, bind to CHS to enhance THC production and decrease CTAL formation, thereby rectifying the promiscuous CHS catalysis. This CHIL function has been confirmed in diverse land plant species, and represents a conserved strategy facilitating the efficient influx of substrates from the phenylpropanoid pathway to the flavonoid pathway. |
| 巻・号 | 11(1) |
| ページ | 870 |
| 公開日 | 2020-2-13 |
| DOI | 10.1038/s41467-020-14558-9 |
| PII | 10.1038/s41467-020-14558-9 |
| PMID | 32054839 |
| PMC | PMC7018950 |
| MeSH | Acyltransferases / genetics Acyltransferases / metabolism* Arabidopsis / genetics Arabidopsis / metabolism Biocatalysis Biosynthetic Pathways / genetics Chalcones / biosynthesis Embryophyta / genetics Embryophyta / metabolism* Evolution, Molecular Flavonoids / biosynthesis Genes, Plant Genetic Complementation Test Intramolecular Lyases / genetics Intramolecular Lyases / metabolism* Kinetics Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Polyketides / metabolism Substrate Specificity |
| IF | 12.121 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| アサガオ | EST clones |