RRC ID 58415
著者 Waki T, Mameda R, Nakano T, Yamada S, Terashita M, Ito K, Tenma N, Li Y, Fujino N, Uno K, Yamashita S, Aoki Y, Denessiouk K, Kawai Y, Sugawara S, Saito K, Yonekura-Sakakibara K, Morita Y, Hoshino A, Takahashi S, Nakayama T.
タイトル A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity.
ジャーナル Nat Commun
Abstract Land plants produce diverse flavonoids for growth, survival, and reproduction. Chalcone synthase is the first committed enzyme of the flavonoid biosynthetic pathway and catalyzes the production of 2',4,4',6'-tetrahydroxychalcone (THC). However, it also produces other polyketides, including p-coumaroyltriacetic acid lactone (CTAL), because of the derailment of the chalcone-producing pathway. This promiscuity of CHS catalysis adversely affects the efficiency of flavonoid biosynthesis, although it is also believed to have led to the evolution of stilbene synthase and p-coumaroyltriacetic acid synthase. In this study, we establish that chalcone isomerase-like proteins (CHILs), which are encoded by genes that are ubiquitous in land plant genomes, bind to CHS to enhance THC production and decrease CTAL formation, thereby rectifying the promiscuous CHS catalysis. This CHIL function has been confirmed in diverse land plant species, and represents a conserved strategy facilitating the efficient influx of substrates from the phenylpropanoid pathway to the flavonoid pathway.
巻・号 11(1)
ページ 870
公開日 2020-2-13
DOI 10.1038/s41467-020-14558-9
PII 10.1038/s41467-020-14558-9
PMID 32054839
PMC PMC7018950
MeSH Acyltransferases / genetics Acyltransferases / metabolism* Arabidopsis / genetics Arabidopsis / metabolism Biocatalysis Biosynthetic Pathways / genetics Chalcones / biosynthesis Embryophyta / genetics Embryophyta / metabolism* Evolution, Molecular Flavonoids / biosynthesis Genes, Plant Genetic Complementation Test Intramolecular Lyases / genetics Intramolecular Lyases / metabolism* Kinetics Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Polyketides / metabolism Substrate Specificity
IF 12.121
引用数 0
リソース情報
アサガオ EST clones