RRC ID 58471
Author Miyazaki K, Tomariguchi N.
Title Occurrence of randomly recombined functional 16S rRNA genes in Thermus thermophilus suggests genetic interoperability and promiscuity of bacterial 16S rRNAs.
Journal Sci Rep
Abstract Based on the structural complexity of ribosomes, 16S rRNA genes are considered species-specific and hence used for bacterial phylogenetic analysis. However, a growing number of reports suggest the occurrence of horizontal gene transfer, raising genealogical questions. Here we show the genetic interoperability and promiscuity of 16S rRNA in the ribosomes of an extremely thermophilic bacterium, Thermus thermophilus. The gene in this thermophile was systematically replaced with a diverse array of heterologous genes, resulting in the discovery of various genes that supported growth, some of which were from different phyla. Moreover, numerous functional chimeras were spontaneously generated. Remarkably, cold-adapted mutants were obtained carrying chimeric or full-length heterologous genes, indicating that horizontal gene transfer promoted adaptive evolution. The ribosome may well be understood as a patchworked supramolecule comprising patchworked components. We here propose the "random patch model" for ribosomal evolution.
Volume 9(1)
Pages 11233
Published 2019-8-2
DOI 10.1038/s41598-019-47807-z
PII 10.1038/s41598-019-47807-z
PMID 31375780
PMC PMC6677816
IF 4.011
Times Cited 1
Resource
DNA material pUC4KrB1 (RDB17943) pUC4KrA1 (RDB17944) pUC4KrA1Hg (RDB17945)