論文 - 詳細
| RRC ID | 58504 |
|---|---|
| 著者 | Kuroishi T, Bando K, Bakti RK, Ouchi G, Tanaka Y, Sugawara S. |
| タイトル | Migratory dendritic cells in skin-draining lymph nodes have nickel-binding capabilities. |
| ジャーナル | Sci Rep |
| Abstract |
Nickel (Ni) is the most frequent metal allergen and induces Th1-dependent type-IV allergies. In local skin, epidermal Langerhans cells (LCs) and/or dermal dendritic cells (DCs) uptake antigens and migrate to draining lymph nodes (LNs). However, the subsets of antigen-presenting cells that contribute to Ni presentation have not yet been identified. In this study, we analyzed the Ni-binding capabilities of murine DCs using fluorescent metal indicator Newport Green. Elicitation of Ni allergy was assessed after intradermal (i.d.) injection of Ni-treated DCs into ear pinnae of Ni-sensitized mice. The Ni-binding capabilities of MHC class IIhi CD11cint migratory DCs were significantly stronger than those of MHC class IIint CD11chi resident DCs and CD11cint PDCA1+ MHC class IIint B220+ plasmacytoid DCs. Migratory DCs in skin-draining and mandibular LNs showed significantly stronger Ni-binding capabilities than those in mesenteric and medial iliac LNs. An i.d. injection of IL-1β induced the activation of LCs and dermal DCs with strong Ni-binding capabilities. Ni-binding LCs were detected in draining LNs after i.d. challenge with IL-1β and Ni. Moreover, an i.d. injection of Ni-treated DCs purified from skin-draining LNs elicited Ni-allergic inflammation. These results demonstrated that migratory DCs in skin-draining LNs have strong Ni-binding capabilities and elicit Ni allergy. |
| 巻・号 | 10(1) |
| ページ | 5050 |
| 公開日 | 2020-3-19 |
| DOI | 10.1038/s41598-020-61875-6 |
| PII | 10.1038/s41598-020-61875-6 |
| PMID | 32193426 |
| PMC | PMC7081353 |
| MeSH | Allergens / immunology Animals CD11 Antigens / immunology Cell Movement / immunology* Cells, Cultured Dendritic Cells / immunology* Dermis / cytology* Dermis / immunology Humans Interleukin-1beta / immunology Mice Nickel / immunology* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00144 |