| RRC ID |
58504
|
| Author |
Kuroishi T, Bando K, Bakti RK, Ouchi G, Tanaka Y, Sugawara S.
|
| Title |
Migratory dendritic cells in skin-draining lymph nodes have nickel-binding capabilities.
|
| Journal |
Sci Rep
|
| Abstract |
Nickel (Ni) is the most frequent metal allergen and induces Th1-dependent type-IV allergies. In local skin, epidermal Langerhans cells (LCs) and/or dermal dendritic cells (DCs) uptake antigens and migrate to draining lymph nodes (LNs). However, the subsets of antigen-presenting cells that contribute to Ni presentation have not yet been identified. In this study, we analyzed the Ni-binding capabilities of murine DCs using fluorescent metal indicator Newport Green. Elicitation of Ni allergy was assessed after intradermal (i.d.) injection of Ni-treated DCs into ear pinnae of Ni-sensitized mice. The Ni-binding capabilities of MHC class IIhi CD11cint migratory DCs were significantly stronger than those of MHC class IIint CD11chi resident DCs and CD11cint PDCA1+ MHC class IIint B220+ plasmacytoid DCs. Migratory DCs in skin-draining and mandibular LNs showed significantly stronger Ni-binding capabilities than those in mesenteric and medial iliac LNs. An i.d. injection of IL-1β induced the activation of LCs and dermal DCs with strong Ni-binding capabilities. Ni-binding LCs were detected in draining LNs after i.d. challenge with IL-1β and Ni. Moreover, an i.d. injection of Ni-treated DCs purified from skin-draining LNs elicited Ni-allergic inflammation. These results demonstrated that migratory DCs in skin-draining LNs have strong Ni-binding capabilities and elicit Ni allergy.
|
| Volume |
10(1)
|
| Pages |
5050
|
| Published |
2020-3-19
|
| DOI |
10.1038/s41598-020-61875-6
|
| PII |
10.1038/s41598-020-61875-6
|
| PMID |
32193426
|
| PMC |
PMC7081353
|
| MeSH |
Allergens / immunology
Animals
CD11 Antigens / immunology
Cell Movement / immunology*
Cells, Cultured
Dendritic Cells / immunology*
Dermis / cytology*
Dermis / immunology
Humans
Interleukin-1beta / immunology
Mice
Nickel / immunology*
|
| IF |
3.998
|
| Resource |
| Mice |
RBRC00144 |
