| RRC ID |
58591
|
| Author |
Fujihara Y, Abe T, Asawa Y, Nishizawa S, Saijo H, Hikita A, Hoshi K.
|
| Title |
Influence of Damage-Associated Molecular Patterns from Chondrocytes in Tissue-Engineered Cartilage.
|
| Journal |
Tissue Eng Part A
|
| Abstract |
To obtain stable outcomes in regenerative medicine, the quality of cells for transplantation is of great importance. Cellular stress potentially results in the release of damage-associated molecular patterns (DAMPs) and activates immunological responses, affecting the outcome of transplanted tissue. In this study, we intentionally prepared necrotic chondrocytes that would gradually die and release DAMPs and investigated how the maturation of tissue-engineered cartilage was affected. Necrotic chondrocytes were prepared by a conventional heat-treatment method, by which their viability started to decrease after 24 h. When tissue-engineered cartilage containing necrotic chondrocytes was subcutaneously transplanted into C57BL/6J mice, accumulation of cartilage matrix was decreased compared to the control. Meanwhile, immunohistochemical staining demonstrated that localization of macrophages and neutrophils was more apparent in the constructs of necrotic chondrocytes, suggesting that DAMPs from necrotic chondrocytes could prompt migration of more immune cells. Two-dimensional electrophoresis and mass spectrometry identified prelamin as a significant biomolecule released from necrotic chondrocytes. Also, when prelamin was added to a culture of RAW264, Inos and Il1b were increased in accordance with the content of added prelamin. It was suggested that DAMPs from dying chondrocytes could induce inflammatory properties in surrounding macrophages, impairing the maturation of tissue-engineered cartilage. In conclusion, maturation of tissue-engineered cartilage was hampered when less viable chondrocytes releasing DAMPs were included.
|
| Volume |
27(1-2)
|
| Pages |
1-9
|
| Published |
2021-1-1
|
| DOI |
10.1089/ten.TEA.2019.0185
|
| PMID |
31724485
|
| MeSH |
Animals
Cartilage*
Cells, Cultured
Chondrocytes*
Macrophages
Mice
Mice, Inbred C57BL
Tissue Engineering
|
| IF |
3.508
|
| Times Cited |
0
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
