RRC ID 5867
Author Xiao JZ, Takahashi S, Nishimoto M, Odamaki T, Yaeshima T, Iwatsuki K, Kitaoka M.
Title Distribution of in vitro fermentation ability of lacto-N-biose I, a major building block of human milk oligosaccharides, in bifidobacterial strains.
Journal Appl Environ Microbiol
Abstract This study investigated the potential utilization of lacto-N-biose I (LNB) by individual strains of bifidobacteria. LNB is a building block for the human milk oligosaccharides, which have been suggested to be a factor for selective growth of bifidobacteria. A total of 208 strains comprising 10 species and 4 subspecies were analyzed for the presence of the galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) gene (lnpA) and examined for growth when LNB was used as the sole carbohydrate source. While all strains of Bifidobacterium longum subsp. longum, B. longum subsp. infantis, B. breve, and B. bifidum were able to grow on LNB, none of the strains of B. adolescentis, B. catenulatum, B. dentium, B. angulatum, B. animalis subsp. lactis, and B. thermophilum showed any growth. In addition, some strains of B. pseudocatenulatum, B. animalis subsp. animalis, and B. pseudolongum exhibited the ability to utilize LNB. With the exception for B. pseudocatenulatum, the presence of lnpA coincided with LNB utilization in almost all strains. These results indicate that bifidobacterial species, which are the predominant species found in infant intestines, are potential utilizers of LNB. These findings support the hypothesis that GLNBP plays a key role in the colonization of bifidobacteria in the infant intestine.
Volume 76(1)
Pages 54-9
Published 2010-1-1
DOI 10.1128/AEM.01683-09
PII AEM.01683-09
PMID 19854932
PMC PMC2798660
MeSH Acetylglucosamine / analogs & derivatives* Acetylglucosamine / metabolism Bacterial Proteins / genetics Bifidobacterium / enzymology Bifidobacterium / genetics Bifidobacterium / growth & development Bifidobacterium / metabolism* Fermentation Humans Milk, Human / chemistry* Oligosaccharides / metabolism
IF 4.016
Times Cited 71
General Microbes JCM 7130 JCM 7040 JCM 7135 JCM 1253