論文 - 詳細
| RRC ID | 58690 |
|---|---|
| 著者 | Wu Y, Zhou Y, Huang CJ, Kobayashi H, Yan S, Ozeki Y, Wu Y, Sun CW, Yasumoto A, Yatomi Y, Lei C, Goda K. |
| タイトル | Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging. |
| ジャーナル | Opt Express |
| Abstract |
Optofluidic time-stretch quantitative phase imaging (OTS-QPI) is a powerful tool as it enables high-throughput (>10,000 cell/s) QPI of single live cells. OTS-QPI is based on decoding temporally stretched spectral interferograms that carry the spatial profiles of cells flowing on a microfluidic chip. However, the utility of OTS-QPI is troubled by difficulties in phase retrieval from the high-frequency region of the temporal interferograms, such as phase-unwrapping errors, high instrumentation cost, and large data volume. To overcome these difficulties, we propose and experimentally demonstrate frequency-shifted OTS-QPI by bringing the phase information to the baseband region. Furthermore, to show its boosted utility, we use it to demonstrate image-based classification of leukemia cells with high accuracy over 96% and evaluation of drug-treated leukemia cells via deep learning. |
| 巻・号 | 28(1) |
| ページ | 519-532 |
| 公開日 | 2020-1-6 |
| DOI | 10.1364/OE.380679 |
| PII | 425575 |
| PMID | 32118978 |
| MeSH | Antineoplastic Agents / pharmacology Antineoplastic Agents / therapeutic use HL-60 Cells Humans Imaging, Three-Dimensional* K562 Cells Leukemia / drug therapy Leukemia / pathology Microfluidics* Optics and Photonics* Time Factors |
| IF | 3.669 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL-60(RCB3683), K562 |