論文 - 詳細
| RRC ID | 58702 |
|---|---|
| 著者 | Kasai T, Suga H, Sakakibara M, Ozone C, Matsumoto R, Kano M, Mitsumoto K, Ogawa K, Kodani Y, Nagasaki H, Inoshita N, Sugiyama M, Onoue T, Tsunekawa T, Ito Y, Takagi H, Hagiwara D, Iwama S, Goto M, Banno R, Takahashi J, Arima H. |
| タイトル | Hypothalamic Contribution to Pituitary Functions Is Recapitulated In Vitro Using 3D-Cultured Human iPS Cells. |
| ジャーナル | Cell Rep |
| Abstract |
The pituitary is a major hormone center that secretes systemic hormones responding to hypothalamus-derived-releasing hormones. Previously, we reported the independent pituitary induction and hypothalamic differentiation of human embryonic stem cells (ESCs). Here, a functional hypothalamic-pituitary unit is generated using human induced pluripotent stem (iPS) cells in vitro. The adrenocorticotropic hormone (ACTH) secretion capacity of the induced pituitary reached a comparable level to that of adult mouse pituitary because of the simultaneous maturation with hypothalamic neurons within the same aggregates. Corticotropin-releasing hormone (CRH) from the hypothalamic area regulates ACTH cells similarly to our hypothalamic-pituitary axis. Our induced hypothalamic-pituitary units respond to environmental hypoglycemic condition in vitro, which mimics a life-threatening situation in vivo, through the CRH-ACTH pathway, and succeed in increasing ACTH secretion. Thus, we generated powerful hybrid organoids by recapitulating hypothalamic-pituitary development, showing autonomous maturation on the basis of interactions between developing tissues. |
| 巻・号 | 30(1) |
| ページ | 18-24.e5 |
| 公開日 | 2020-1-7 |
| DOI | 10.1016/j.celrep.2019.12.009 |
| PII | S2211-1247(19)31648-1 |
| PMID | 31914385 |
| MeSH | Adrenocorticotropic Hormone / metabolism Aging / physiology Animals Cell Differentiation Cells, Cultured Corticotrophs / cytology Corticotrophs / ultrastructure Humans Hypothalamus / physiology* Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / ultrastructure Mice Neurons / cytology Organoids / cytology Pituitary Gland / physiology* |
| IF | 8.109 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 409B2(HPS0076) 454E2(HPS0077) |