| RRC ID |
5871
|
| 著者 |
Khelifi N, Ben Romdhane E, Hedi A, Postec A, Fardeau ML, Hamdi M, Tholozan JL, Ollivier B, Hirschler-Réa A.
|
| タイトル |
Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia.
|
| ジャーナル |
Extremophiles
|
| Abstract |
A novel thermophilic anaerobic and microaerophilic bacterium (optimal growth in the presence of 5-10% O(2)), strain Nad S1(T) was isolated from the terrestrial hot spring of Hammam Sidi Jdidi, Nabeul, Tunisia. Cells were motile rods having a Gram-positive cell wall structure. Strain Nad S1(T) grew optimally at 55 degrees C (range 37-70 degrees C). Optimum pH for growth was 6.5-7.0. It was halotolerant growing with NaCl up to 7% (optimum concentration 1.5-3.0%). It grew chemoorganotrophically on various carbohydrates, organic-acids and amino-acids as energy sources, or chemolithotrophically on H(2) using nitrate, as terminal electron acceptor. Beside oxygen (under microaerobic conditions) and nitrate, nitrite was also used. Nitrate was completely reduced to N(2). No fermentation occurred. The genomic DNA G + C content was 41.8 mol%. Based on 16S rRNA gene sequence analysis, strain Nad S1(T) belongs to the Bacillaceae family within the class 'Bacilli'. Because of its phylogenetic and phenotypic characteristics, we propose this isolate to be assigned as a novel genus and a novel species within the domain Bacteria, Microaerobacter geothermalis gen. nov., sp. nov. The type strain is Nad S1(T) (=DSM 22679(T) =JCM 16213(T)).
|
| 巻・号 |
14(3)
|
| ページ |
297-304
|
| 公開日 |
2010-5-1
|
| DOI |
10.1007/s00792-010-0308-5
|
| PMID |
20358237
|
| MeSH |
Bacteria / classification*
Bacteria / metabolism
Base Composition
Cell Wall / metabolism
Hot Springs
Hydrogen-Ion Concentration
Microscopy, Electron / methods
Microscopy, Phase-Contrast
Models, Genetic
Nitrates / chemistry*
Nitrites / chemistry*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / chemistry
Temperature
Tunisia
|
| IF |
2.462
|
| 引用数 |
16
|
|
WOS 分野
|
MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 16213 |
