論文 - 詳細
| RRC ID | 58717 |
|---|---|
| 著者 | Bandow K, Hasegawa H, Tomomura M, Tomomura A. |
| タイトル | Caldecrin inhibits lipopolysaccharide-induced pro-inflammatory cytokines and M1 macrophage polarization through the immunoreceptor triggering receptor expressed in myeloid cells-2. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Caldecrin was previously isolated as a serum calcium-decreasing factor from the pancreas and is known to suppress receptor activator of nuclear factor-κB ligand (RANKL)-induced calcium oscillation pathways in osteoclasts. Here, we explored the effects of caldecrin on lipopolysaccharide (LPS)-Toll-like receptor-4 (TLR-4) signaling pathways in macrophages. Caldecrin inhibited the LPS-induced gene expression of pro-inflammatory cytokines and M1 macrophage polarization in mouse bone marrow macrophages and the RAW264.7 mouse macrophage cell line. Next, we focused on triggering receptor expressed in myeloid cells-2 (TREM-2) as a co-receptor common to RANKL receptor and TLR-4, and established Trem2-KO RAW264.7 cells, in which Trem2 gene was deleted using the CRISPR/Cas9 system. Caldecrin-mediated alterations in pro-inflammatory cytokine expression and M1 macrophage polarization were not observed in Trem2-KO RAW264.7 cells. These results suggest that caldecrin is not only an inhibitor of osteoclast activation but also a negative regulator of LPS-induced inflammatory responses, functioning via TREM-2. |
| 巻・号 | 523(4) |
| ページ | 1027-1033 |
| 公開日 | 2020-3-19 |
| DOI | 10.1016/j.bbrc.2020.01.045 |
| PII | S0006-291X(20)30108-X |
| PMID | 31973822 |
| MeSH | Animals Base Sequence Cell Polarity Cytokines / genetics Cytokines / metabolism* Gene Expression Regulation / drug effects Inflammation Mediators / metabolism* Lipopolysaccharides / pharmacology* Macrophages / cytology* Macrophages / drug effects Macrophages / metabolism* Membrane Glycoproteins / metabolism* Mice RAW 264.7 Cells Receptors, Immunologic / metabolism* Serine Endopeptidases / metabolism* Signal Transduction / drug effects |
| IF | 2.985 |
| 引用数 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |