Reference - Detail
| RRC ID | 58726 |
|---|---|
| Author | Komatsu T, Shimizu T, Kanoh M, Miyakawa T, Satta Y, Yasukochi Y, Fujimoto R, Tada M, Machida K, Kataoka S, Udaka K. |
| Title | Development of a novel monoclonal antibody that binds to most HLA-A allomorphs in a conformation-dependent yet peptide-promiscuous fashion. |
| Journal | Immunogenetics |
| Abstract |
Specificity analyses of peptide binding to human leukocyte antigen (HLA)-A molecules have been hampered due to a lack of proper monoclonal antibodies (mAbs) for certain allomorphs, such as the prevalent HLA-A1 for Caucasians and HLA-A11 for Asians. We developed a mAb that recognizes a conformational epitope common to most HLA-A allomorphs. The mAb, named A-1, does not discriminate peptides by amino acid sequences, making it suitable for measuring peptide binding. A stabilization assay using TAP-deficient cell lines and A-1 was developed to investigate the specificity of peptide binding to HLA-A molecules. Regarding the evolution of HLA-A genes, the A-1 epitope has been conserved among most HLA-A allomorphs but was lost when the HLA-A gene diversified into the HLA-A*32, HLA-A*31, and HLA-A*33 lineages together with HLA-A*29 after bifurcating from the HLA-A*25 and HLA-A*26 branchs. The establishment of A-1 is expected to help researchers investigate the peptide repertoire and develop computational tools to identify cognate peptides. Since no HLA-A locus-specific mAb has been available, A-1 will also be useful for analyzing the locus-specific regulation of the HLA gene expression. |
| Volume | 72(3) |
| Pages | 143-153 |
| Published | 2020-4-1 |
| DOI | 10.1007/s00251-020-01154-w |
| PII | 10.1007/s00251-020-01154-w |
| PMID | 31970435 |
| MeSH | Amino Acid Sequence Antibodies, Monoclonal / immunology Antibodies, Monoclonal / metabolism* Cell Line, Tumor Epitopes / immunology HLA-A Antigens / chemistry HLA-A Antigens / immunology* HLA-A1 Antigen / chemistry HLA-A1 Antigen / immunology* Humans Models, Molecular Peptides / immunology Protein Binding / immunology Protein Conformation |
| IF | 2.621 |
| Times Cited | 0 |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HEV0400 HEV0012 |