論文 - 詳細
| RRC ID | 58743 |
|---|---|
| 著者 | Konagaya S, Iwata H. |
| タイトル | Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
Large numbers of hormone-releasing cells, approximately 109 endocrine cells, are required to treat type I diabetes patients by cell transplantation. The SOX9-positive pancreatic epithelium proliferates extensively during the early stages of pancreatic development. SOX9-positive pancreatic epithelium is thought to be an expandable cell source of β cells for transplantation therapy. In this study, we attempted to expand pancreatic progenitors (PPs: PDX1+/SOX9+) derived from four human iPSC lines in three-dimensional (3D) culture using a chemically defined medium and examined the potential of the derived PPs to differentiate into β-like cells. PPs from four human iPSC lines were maintained and effectively proliferated in a chemically defined medium containing epidermal growth factor and R-spondin-1, CHIR99021, fibroblast growth factor-7, and SB431542. PPs derived from one iPSC line can be expanded by more than 104-fold in chemically defined medium containing two of the fives, epidermal growth factor and R-spondin-1. The expanded PPs were also stable following cryopreservation. After freezing and thawing, the PPs proliferated without a decrease in the rate. PPs obtained after 50 days of culture successfully differentiated into insulin-positive β-like cells, glucagon-positive α-like cells, and somatostatin-positive δ-like cells. The differentiation efficiency of expanded PPs was similar to that of PPs without expansion culture. |
| 巻・号 | 9(1) |
| ページ | 640 |
| 公開日 | 2019-1-24 |
| DOI | 10.1038/s41598-018-36606-7 |
| PII | 10.1038/s41598-018-36606-7 |
| PMID | 30679498 |
| PMC | PMC6345937 |
| MeSH | Benzamides / pharmacology Cell Differentiation / drug effects Cell Proliferation / drug effects Dioxoles / pharmacology Endocrine Cells / cytology Endocrine Cells / drug effects Epidermal Growth Factor / pharmacology Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Insulin-Secreting Cells / cytology Insulin-Secreting Cells / drug effects Pancreas / cytology* Pyridines / pharmacology Pyrimidines / pharmacology |
| IF | 3.998 |
| 引用数 | 3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |