RRC ID 58743
著者 Konagaya S, Iwata H.
タイトル Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells.
ジャーナル Sci Rep
Abstract Large numbers of hormone-releasing cells, approximately 109 endocrine cells, are required to treat type I diabetes patients by cell transplantation. The SOX9-positive pancreatic epithelium proliferates extensively during the early stages of pancreatic development. SOX9-positive pancreatic epithelium is thought to be an expandable cell source of β cells for transplantation therapy. In this study, we attempted to expand pancreatic progenitors (PPs: PDX1+/SOX9+) derived from four human iPSC lines in three-dimensional (3D) culture using a chemically defined medium and examined the potential of the derived PPs to differentiate into β-like cells. PPs from four human iPSC lines were maintained and effectively proliferated in a chemically defined medium containing epidermal growth factor and R-spondin-1, CHIR99021, fibroblast growth factor-7, and SB431542. PPs derived from one iPSC line can be expanded by more than 104-fold in chemically defined medium containing two of the fives, epidermal growth factor and R-spondin-1. The expanded PPs were also stable following cryopreservation. After freezing and thawing, the PPs proliferated without a decrease in the rate. PPs obtained after 50 days of culture successfully differentiated into insulin-positive β-like cells, glucagon-positive α-like cells, and somatostatin-positive δ-like cells. The differentiation efficiency of expanded PPs was similar to that of PPs without expansion culture.
巻・号 9(1)
ページ 640
公開日 2019-1-24
DOI 10.1038/s41598-018-36606-7
PII 10.1038/s41598-018-36606-7
PMID 30679498
PMC PMC6345937
MeSH Benzamides / pharmacology Cell Differentiation / drug effects Cell Proliferation / drug effects Dioxoles / pharmacology Endocrine Cells / cytology Endocrine Cells / drug effects Epidermal Growth Factor / pharmacology Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Insulin-Secreting Cells / cytology Insulin-Secreting Cells / drug effects Pancreas / cytology* Pyridines / pharmacology Pyrimidines / pharmacology
IF 3.998
引用数 3
リソース情報
ヒト・動物細胞 253G1(HPS0002)