Reference - Detail
| RRC ID | 58749 |
|---|---|
| Author | Iwahasi S, Rui F, Morine Y, Yamada S, Saito YU, Ikemoto T, Imura S, Shimada M. |
| Title | Hepatic Stellate Cells Contribute to the Tumor Malignancy of Hepatocellular Carcinoma Through the IL-6 Pathway. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:The hepatic stellate cells (HSCs) have relationship to cancer progression. The aim of this study is to investigate the effect of HSCs and the role of IL-6/Stat3 pathway on hepatocellular carcinoma (HCC) progression. MATERIALS AND METHODS:HCCs were co-cultured with HSCs. The viability and migration ability of cancer cells were detected. Epithelial-mesenchymal transition (EMT) marker (E-cadherin), stem cell marker (CD44) and p-signal transducer and activator of transcription 3 (p-STAT3) of cancer cells were evaluated. Finally, interleukin-6 (IL-6) neutralization was performed. RESULTS:Co-culture of HCCs with HSCs increased cancer cell viability and migration ability. EMT and stemness of cancer cells increased with HSCs. Following IL-6 neutralization, phospho-STAT3 activation, cancer cell viability and migration, as well as EMT, and stemness of cancer cells decreased. CONCLUSION:HSCs promoted HCC progression through the IL-6/STAT3 pathway. |
| Volume | 40(2) |
| Pages | 743-749 |
| Published | 2020-2-1 |
| DOI | 10.21873/anticanres.14005 |
| PII | 40/2/743 |
| PMID | 32014916 |
| MeSH | Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Communication / physiology Cell Line, Tumor Cell Movement / physiology Cell Survival / physiology Coculture Techniques Hep G2 Cells Hepatic Stellate Cells / metabolism* Hepatic Stellate Cells / pathology Humans Interleukin-6 / metabolism* Liver Neoplasms / metabolism* Liver Neoplasms / pathology Neoplastic Stem Cells / metabolism Neoplastic Stem Cells / pathology STAT3 Transcription Factor / metabolism Signal Transduction |
| IF | 1.994 |
| Times Cited | 0 |
| Resource | |
| Human and Animal Cells | Hep G2, HuH-7 |