| RRC ID |
58757
|
| 著者 |
Itoh T, Fukatani K, Nakashima A, Suzuki K.
|
| タイトル |
MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor.
|
| ジャーナル |
Sci Rep
|
| Abstract |
In recent years, it has been reported that non-coding RNAs, especially microRNAs (miRNAs) and long non-coding RNAs, act as melanogenesis-regulating molecules in melanocytes. We found that the expression levels of miR-141-3p and miR-200a-3p were decreased significantly by α-melanocyte-stimulating hormone (α-MSH) stimulation in mouse melanocyte B16-4A5 cells, as demonstrated by a miRNA array. Overexpression of miR-141-3p and miR-200a-3p in B16-4A5 cells suppressed melanogenesis and tyrosinase activity. Moreover, both miR-141-3p and miR-200a-3p showed direct targeting of microphthalmia-associated transcription factor using a luciferase reporter assay. Furthermore, topical transfection of miR-141-3p and miR-200a-3p to three-dimensional reconstructed human skin tissue inhibited α-MSH-stimulated melanin biosynthesis. Taken together, our findings indicate that downregulation of miR-141-3p and miR-200a-3p during the α-MSH-stimulated melanogenesis process acts as an important intrinsic signal. This result is expected to lead to the development of miRNA-based whitening therapeutics.
|
| 巻・号 |
10(1)
|
| ページ |
2149
|
| 公開日 |
2020-2-7
|
| DOI |
10.1038/s41598-020-58911-w
|
| PII |
10.1038/s41598-020-58911-w
|
| PMID |
32034251
|
| PMC |
PMC7005774
|
| MeSH |
Animals
Cell Line, Tumor
Cells, Cultured
Humans
Melanins / biosynthesis*
Melanocytes / drug effects
Melanocytes / metabolism
Mice
MicroRNAs / genetics
MicroRNAs / metabolism*
Microphthalmia-Associated Transcription Factor / genetics*
Microphthalmia-Associated Transcription Factor / metabolism
Skin / drug effects
Skin / metabolism
alpha-MSH / pharmacology
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma 4A5(RCB0557) |
