論文 - 詳細
| RRC ID | 58757 |
|---|---|
| 著者 | Itoh T, Fukatani K, Nakashima A, Suzuki K. |
| タイトル | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor. |
| ジャーナル | Sci Rep |
| Abstract |
In recent years, it has been reported that non-coding RNAs, especially microRNAs (miRNAs) and long non-coding RNAs, act as melanogenesis-regulating molecules in melanocytes. We found that the expression levels of miR-141-3p and miR-200a-3p were decreased significantly by α-melanocyte-stimulating hormone (α-MSH) stimulation in mouse melanocyte B16-4A5 cells, as demonstrated by a miRNA array. Overexpression of miR-141-3p and miR-200a-3p in B16-4A5 cells suppressed melanogenesis and tyrosinase activity. Moreover, both miR-141-3p and miR-200a-3p showed direct targeting of microphthalmia-associated transcription factor using a luciferase reporter assay. Furthermore, topical transfection of miR-141-3p and miR-200a-3p to three-dimensional reconstructed human skin tissue inhibited α-MSH-stimulated melanin biosynthesis. Taken together, our findings indicate that downregulation of miR-141-3p and miR-200a-3p during the α-MSH-stimulated melanogenesis process acts as an important intrinsic signal. This result is expected to lead to the development of miRNA-based whitening therapeutics. |
| 巻・号 | 10(1) |
| ページ | 2149 |
| 公開日 | 2020-2-7 |
| DOI | 10.1038/s41598-020-58911-w |
| PII | 10.1038/s41598-020-58911-w |
| PMID | 32034251 |
| PMC | PMC7005774 |
| MeSH | Animals Cell Line, Tumor Cells, Cultured Humans Melanins / biosynthesis* Melanocytes / drug effects Melanocytes / metabolism Mice MicroRNAs / genetics MicroRNAs / metabolism* Microphthalmia-Associated Transcription Factor / genetics* Microphthalmia-Associated Transcription Factor / metabolism Skin / drug effects Skin / metabolism alpha-MSH / pharmacology |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma 4A5(RCB0557) |