論文 - 詳細
| RRC ID | 58781 |
|---|---|
| 著者 | Hata K, Sayaka T, Takahashi M, Sasaki A, Umekawa Y, Miyashita K, Ogura K, Toshima G, Maeda M, Takahashi J, Kakuni M. |
| タイトル | Lipoprotein profile and lipid metabolism of PXB-cells®, human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs. |
| ジャーナル | Biomed Res |
| Abstract |
We investigated lipid metabolism in PXB-cells, which are human primary hepatocytes isolated from liver-humanized mice, and HepG2 and HuH-7 human hepatoma cell lines. Lipoprotein levels were higher in PXB-cells than in the 2 other cell lines, and PXB-cells mainly released triglycerides and cholesterol as very low density lipoprotein (VLDL), similar to actual liver tissue, whereas the major lipoprotein released from the 2 hepatoma cell lines was LDL. RT-PCR analysis demonstrated that the gene expression levels of apolipoprotein B100 (ApoB100), the apolipoprotein of VLDL/LDL, were similar in PXB-cells and HepG2 cells, while the overexpression of ApoC2, ApoC3, and ApoE, which are components of VLDL, but not LDL, was observed in PXBcells. A protein immunoassay revealed that ApoB100 levels secreted from PXB-cells and HuH-7 cells were similar; however, ApoC3 levels were higher in PXB-cells than in the two other cell lines. We also examined the anti-lipidemic activities of fenofibrate using this assay system. Fenofibrate suppressed lipoprotein production from PXB-cells in a dose-dependent manner mainly by activating the β-oxidation pathway. These results suggest that PXB-cells produce high levels of lipoproteins and are suitable for screening anti-lipidemic agents. |
| 巻・号 | 41(1) |
| ページ | 33-42 |
| 公開日 | 2020-1-1 |
| DOI | 10.2220/biomedres.41.33 |
| PMID | 32092738 |
| MeSH | Animals Apolipoprotein B-100 / metabolism Carcinoma, Hepatocellular / metabolism Cell Line, Tumor Fenofibrate / pharmacology* Hep G2 Cells Hepatocytes Humans Lipid Metabolism* Lipoproteins / metabolism* Lipoproteins, VLDL / metabolism Liver / metabolism Liver / pathology Liver Neoplasms / metabolism Mice Mice, SCID Oxygen / chemistry Time Factors Triglycerides / metabolism |
| IF | 1.429 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2, HuH-7 |