論文 - 詳細
| RRC ID | 58804 |
|---|---|
| 著者 | Noda S, Chikazawa K, Suzuki Y, Imaizumi Y, Yamamura H. |
| タイトル | Involvement of the γ1 subunit of the large-conductance Ca2+-activated K+ channel in the proliferation of human somatostatinoma cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Pancreatic neuroendocrine tumors (pNETs) occur due to the abnormal growth of pancreatic islet cells and predominantly develop in the duodenal-pancreatic region. Somatostatinoma is one of the pNETs associated with tumors of pancreatic δ cells, which produce and secrete somatostatin. Limited information is currently available on the pathogenic mechanisms of somatostatinoma. The large-conductance Ca2+-activated K+ (BKCa) channel is expressed in several types of cancer cells and regulates cell proliferation, migration, invasion, and metastasis. In the present study, the functional expression of the BKCa channel was examined in a human somatostatinoma QGP-1 cell line. In QGP-1 cells, outward currents were elicited by membrane depolarization at pCa 6.5 (300 nM) in the pipette solution and inhibited by the specific BKCa channel blocker, paxilline. Paxilline-sensitive currents were detected, even at pCa 8.0 (10 nM) in the pipette solution, in QGP-1 cells. In addition to the α and β2-4 subunits of the BKCa channel, the novel regulatory γ1 subunit (BKCaγ1) was co-localized with the α subunit in QGP-1 cells. Paxilline-sensitive currents at pCa 8.0 in the pipette solution were reduced by the siRNA knockdown of BKCaγ1. Store-operated Ca2+ entry was smaller in BKCaγ1 siRNA-treated QGP-1 cells. The proliferation of QGP-1 cells was attenuated by paxilline or the siRNA knockdown of BKCaγ1. These results strongly suggest that BKCaγ1 facilitates the proliferation of human somatostatinoma cells. Therefore, BKCaγ1 may be a novel therapeutic target for somatostatinoma. |
| 巻・号 | 525(4) |
| ページ | 1032-1037 |
| 公開日 | 2020-5-14 |
| DOI | 10.1016/j.bbrc.2020.02.176 |
| PII | S0006-291X(20)30498-8 |
| PMID | 32178873 |
| MeSH | Calcium / metabolism* Cell Line, Tumor Cell Proliferation / genetics Gene Knockdown Techniques Humans Immunohistochemistry Indoles / pharmacology Large-Conductance Calcium-Activated Potassium Channels / genetics Large-Conductance Calcium-Activated Potassium Channels / metabolism* Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* Potassium Channel Blockers / pharmacology RNA, Small Interfering Somatostatinoma / genetics Somatostatinoma / metabolism* |
| IF | 2.985 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) |