論文 - 詳細
| RRC ID | 58806 |
|---|---|
| 著者 | Li Z, Kurosawa O, Iwata H. |
| タイトル | A comparative study of key physiological stem cell parameters between three human trophoblast cell lines. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Human trophoblast stem cells (TSCs) play a key role in the placenta. These cells are proliferative, undifferentiated, and can differentiate into mature trophoblast cell types. However, primary human TSCs are difficult to obtain. In our previous study, we established TSCs from human induced pluripotent stem cells (TShiPSC). Here, we aimed to characterize the identity of these TShiPSC cells by comparing them with BeWo choriocarcinoma cells and primary TSCs (CT cells). Compared with BeWo cells, CT and TShiPSC cells showed high secretion of human chorionic gonadotrophin (hCG) and syncytiotrophoblast differentiation ability. Global gene microarray analysis results showed that CT and TShiPSC cells, unlike BeWo cells, could be classified in the same group. Compared with BeWo cells, CT and TShiPSC cells showed high expression levels of TSC-specific genes and low expression of cancer adhesion and invasion genes. Analysis of placental barrier integrity showed that TShiPSC cells could form a good barrier. Prospective studies using TShiPSC cells hold great promise for elucidating the pathogenesis of infertility due to trophoblast defects. |
| 巻・号 | 525(4) |
| ページ | 1038-1045 |
| 公開日 | 2020-5-14 |
| DOI | 10.1016/j.bbrc.2020.03.031 |
| PII | S0006-291X(20)30510-6 |
| PMID | 32178868 |
| MeSH | Cell Differentiation / drug effects Cell Differentiation / genetics Cell Differentiation / physiology Cell Line, Tumor Choriocarcinoma / genetics Choriocarcinoma / metabolism Chorionic Gonadotropin / metabolism* Female Fluorescent Antibody Technique Gene Expression Profiling Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / physiology Oligonucleotide Array Sequence Analysis Placenta / metabolism Placenta / physiology Pregnancy Stem Cells / cytology* Stem Cells / metabolism Stem Cells / physiology Trophoblasts / cytology* Trophoblasts / metabolism* Trophoblasts / physiology |
| IF | 2.985 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | BeWo(RCB1644) CT27(RCB4936) |