| RRC ID |
58807
|
| 著者 |
Hashimura S, Kido J, Matsuda R, Yokota M, Matsui H, Inoue-Fujiwara M, Inagaki Y, Hidaka M, Tanaka T, Tsutsumi T, Nagata T, Tokumura A.
|
| タイトル |
A low level of lysophosphatidic acid in human gingival crevicular fluid from patients with periodontitis due to high soluble lysophospholipase activity: Its potential protective role on alveolar bone loss by periodontitis.
|
| ジャーナル |
Biochim Biophys Acta Mol Cell Biol Lipids
|
| Abstract |
We previously detected a submicromolar concentration of lysophosphatidic acid (LPA) in human saliva. Here, we compare LPA concentrations in human gingival crevicular fluid (GCF) from patients with periodontitis and healthy controls, and examine how the local LPA levels are regulated enzymatically. The concentrations of LPA and its precursor lysophospholipids in GCF was measured by liquid chromatography-tandem mass spectrometry. The LPA-producing and LPA-degrading enzymatic activities were measured by quantifying the liberated choline and free fatty acid, respectively. The concentration of LPA in GCF of periodontitis patients was lower than that of healthy controls, due to higher soluble lysophospholipase activity toward LPA. LPA was found to prevent survival of Sa3, a human gingival epithelium-derived tumor cell line, activate Sa3 through Ca2+ mobilization, and release interleukin 6 from Sa3 in vitro. Furthermore, local injection of LPA into the gingiva attenuated ligature-induced experimental alveolar bone loss induced by oral bacteria inoculation in a rat model of periodontitis in vivo. A high concentration of LPA in human GCF is necessary to maintain normal gingival epithelial integrity and function, protecting the progression of periodontitis.
|
| 巻・号 |
1865(7)
|
| ページ |
158698
|
| 公開日 |
2020-7-1
|
| DOI |
10.1016/j.bbalip.2020.158698
|
| PII |
S1388-1981(20)30090-1
|
| PMID |
32179099
|
| MeSH |
Adult
Aged
Alveolar Bone Loss / etiology
Alveolar Bone Loss / metabolism*
Alveolar Bone Loss / prevention & control
Animals
Cells, Cultured
Female
Gingival Crevicular Fluid / metabolism*
Humans
Lysophospholipase / metabolism*
Lysophospholipids / metabolism*
Lysophospholipids / therapeutic use
Male
Middle Aged
Periodontitis / complications
Periodontitis / drug therapy
Periodontitis / metabolism*
Rats
Rats, Wistar
|
| IF |
4.402
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
Sa3(RCB0980) |
