RRC ID 58813
著者 Afify SM, Sanchez Calle A, Hassan G, Kumon K, Nawara HM, Zahra MH, Mansour HM, Khayrani AC, Alam MJ, Du J, Seno A, Iwasaki Y, Seno M.
タイトル A novel model of liver cancer stem cells developed from induced pluripotent stem cells.
ジャーナル Br J Cancer
Abstract BACKGROUND:Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown.
METHODS:In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed.
RESULTS:The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers.
CONCLUSIONS:We established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.
巻・号 122(9)
ページ 1378-1390
公開日 2020-4-1
DOI 10.1038/s41416-020-0792-z
PII 10.1038/s41416-020-0792-z
PMID 32203212
PMC PMC7188674
MeSH Animals Carcinogenesis / drug effects* Carcinoma, Hepatocellular / etiology Carcinoma, Hepatocellular / genetics* Carcinoma, Hepatocellular / pathology Cell Differentiation / drug effects Cell Line, Tumor Cell Proliferation / drug effects Cell Self Renewal / drug effects Culture Media, Conditioned / metabolism Culture Media, Conditioned / pharmacology* Humans Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / pathology Liver / drug effects Liver / pathology Liver Neoplasms / etiology Liver Neoplasms / genetics* Liver Neoplasms / pathology Mice Mice, Inbred BALB C Neoplastic Stem Cells / drug effects Signal Transduction / drug effects
IF 5.791
引用数 2
リソース情報
ヒト・動物細胞 HuH-7