| RRC ID |
58826
|
| Author |
Shibata S, Hayashi R, Kudo Y, Okubo T, Imaizumi T, Katayama T, Ishikawa Y, Kobayashi Y, Toga J, Taniguchi Y, Honma Y, Sekiguchi K, Nishida K.
|
| Title |
Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium.
|
| Journal |
Stem Cell Reports
|
| Abstract |
A treatment for intractable diseases is expected to be the replacement of damaged tissues with products from human induced pluripotent stem cells (hiPSCs). Target cell purification is a critical step for realizing hiPSC-based therapy. Here, we found that hiPSC-derived ocular cell types exhibited unique adhesion specificities and growth characteristics on distinct E8 fragments of laminin isoforms (LNE8s): hiPSC-derived corneal epithelial cells (iCECs) and other non-CECs rapidly adhered preferentially to LN332/411/511E8 and LN211E8, respectively, through differential expression of laminin-binding integrins. Furthermore, LN332E8 promoted epithelial cell proliferation but not that of the other eye-related cells, leading to non-CEC elimination by cell competition. Combining these features with magnetic sorting, highly pure iCEC sheets were fabricated. Thus, we established a simple method for isolating iCECs from various hiPSC-derived cells without using fluorescence-activated cell sorting. This study will facilitate efficient manufacture of iCEC sheets for corneal disease treatment and provide insights into target cell-specific scaffold selection.
|
| Volume |
14(4)
|
| Pages |
663-676
|
| Published |
2020-4-14
|
| DOI |
10.1016/j.stemcr.2020.02.008
|
| PII |
S2213-6711(20)30064-3
|
| PMID |
32197114
|
| PMC |
PMC7160305
|
| MeSH |
Cell Adhesion / drug effects
Cell Differentiation / drug effects
Cell Proliferation / drug effects
Cell Separation / methods*
Cells, Cultured
Epithelial Cells / cytology
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Epithelium, Corneal / cytology*
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / drug effects
Integrins / metabolism
Laminin / pharmacology*
Protein Isoforms / pharmacology
|
| IF |
6.032
|
| Times Cited |
0
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063) |
