| RRC ID |
58838
|
| 著者 |
Tsukamoto Y, Akagi T, Akashi M.
|
| タイトル |
Vascularized cardiac tissue construction with orientation by layer-by-layer method and 3D printer.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Herein, we report the fabrication of native organ-like three-dimensional (3D) cardiac tissue with an oriented structure and vascular network using a layer-by-layer (LbL), cell accumulation and 3D printing technique for regenerative medicine and pharmaceutical applications. We firstly evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermoresponsive polymer, by using a robotic dispensing 3D printer. Next, we tried to fabricate orientation-controlled 3D cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and normal human cardiac fibroblasts (NHCF) coated with extracellular matrix (ECM) nanofilms by layer-by-layer technique. These cells were seeded in the fabricated rectangular shape HBC gel frame. After cultivation of the fabricated tissue, fluorescence staining of the cytoskeleton revealed that hiPSC-CM and NHCF were aligned in one direction. Moreover, we were able to measure its contractile behavior using a video image analysis system. These results indicate that orientation-controlled cardiac tissue has more remarkable contractile function than uncontrolled cardiac tissue. Finally, co-culture with human cardiac microvascular endothelial cells (HMVEC) successfully provided a vascular network in orientation-controlled 3D cardiac tissue. The constructed 3D cardiac tissue with an oriented structure and vascular network would be a useful tool for regenerative medicine and pharmaceutical applications.
|
| 巻・号 |
10(1)
|
| ページ |
5484
|
| 公開日 |
2020-3-26
|
| DOI |
10.1038/s41598-020-59371-y
|
| PII |
10.1038/s41598-020-59371-y
|
| PMID |
32218447
|
| PMC |
PMC7098983
|
| MeSH |
Bioprosthesis*
Cells, Cultured
Chitosan / analogs & derivatives
Chitosan / chemistry
Coculture Techniques
Coronary Vessels / cytology
Coronary Vessels / physiology
Endothelial Cells / cytology
Fibroblasts / cytology
Heart, Artificial*
Humans
Induced Pluripotent Stem Cells / cytology
Models, Cardiovascular*
Myocardial Contraction / physiology
Myocytes, Cardiac / cytology
Printing, Three-Dimensional*
Regenerative Medicine
Tissue Engineering / methods*
Tissue Scaffolds / chemistry
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
253G1(HPS0002) |
