論文 - 詳細
| RRC ID | 58838 |
|---|---|
| 著者 | Tsukamoto Y, Akagi T, Akashi M. |
| タイトル | Vascularized cardiac tissue construction with orientation by layer-by-layer method and 3D printer. |
| ジャーナル | Sci Rep |
| Abstract |
Herein, we report the fabrication of native organ-like three-dimensional (3D) cardiac tissue with an oriented structure and vascular network using a layer-by-layer (LbL), cell accumulation and 3D printing technique for regenerative medicine and pharmaceutical applications. We firstly evaluated the 3D shaping ability of hydroxybutyl chitosan (HBC), a thermoresponsive polymer, by using a robotic dispensing 3D printer. Next, we tried to fabricate orientation-controlled 3D cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) and normal human cardiac fibroblasts (NHCF) coated with extracellular matrix (ECM) nanofilms by layer-by-layer technique. These cells were seeded in the fabricated rectangular shape HBC gel frame. After cultivation of the fabricated tissue, fluorescence staining of the cytoskeleton revealed that hiPSC-CM and NHCF were aligned in one direction. Moreover, we were able to measure its contractile behavior using a video image analysis system. These results indicate that orientation-controlled cardiac tissue has more remarkable contractile function than uncontrolled cardiac tissue. Finally, co-culture with human cardiac microvascular endothelial cells (HMVEC) successfully provided a vascular network in orientation-controlled 3D cardiac tissue. The constructed 3D cardiac tissue with an oriented structure and vascular network would be a useful tool for regenerative medicine and pharmaceutical applications. |
| 巻・号 | 10(1) |
| ページ | 5484 |
| 公開日 | 2020-3-26 |
| DOI | 10.1038/s41598-020-59371-y |
| PII | 10.1038/s41598-020-59371-y |
| PMID | 32218447 |
| PMC | PMC7098983 |
| MeSH | Bioprosthesis* Cells, Cultured Chitosan / analogs & derivatives Chitosan / chemistry Coculture Techniques Coronary Vessels / cytology Coronary Vessels / physiology Endothelial Cells / cytology Fibroblasts / cytology Heart, Artificial* Humans Induced Pluripotent Stem Cells / cytology Models, Cardiovascular* Myocardial Contraction / physiology Myocytes, Cardiac / cytology Printing, Three-Dimensional* Regenerative Medicine Tissue Engineering / methods* Tissue Scaffolds / chemistry |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |