論文 - 詳細
| RRC ID | 58928 |
|---|---|
| 著者 | Schreiber M, Mascher M, Wright J, Padmarasu S, Himmelbach A, Heavens D, Milne L, Clavijo BJ, Stein N, Waugh R. |
| タイトル | A Genome Assembly of the Barley 'Transformation Reference' Cultivar Golden Promise. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments. |
| 巻・号 | 10(6) |
| ページ | 1823-1827 |
| 公開日 | 2020-4-2 |
| DOI | 10.1534/g3.119.401010 |
| PII | g3.119.401010 |
| PMID | 32241919 |
| PMC | PMC7263683 |
| MeSH | Genome Genomics Genotype High-Throughput Nucleotide Sequencing Hordeum* / genetics |
| IF | 2.781 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| オオムギ | Full lengh cDNA sequences |