RRC ID 58928
著者 Schreiber M, Mascher M, Wright J, Padmarasu S, Himmelbach A, Heavens D, Milne L, Clavijo BJ, Stein N, Waugh R.
タイトル A Genome Assembly of the Barley 'Transformation Reference' Cultivar Golden Promise.
ジャーナル G3 (Bethesda)
Abstract Barley (Hordeum vulgare) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.
巻・号 10(6)
ページ 1823-1827
公開日 2020-4-2
DOI 10.1534/g3.119.401010
PII g3.119.401010
PMID 32241919
PMC PMC7263683
MeSH Genome Genomics Genotype High-Throughput Nucleotide Sequencing Hordeum* / genetics
IF 2.781
引用数 2
リソース情報
オオムギ Full lengh cDNA sequences