RRC ID 58933
Author Abe F, Haque E, Hisano H, Tanaka T, Kamiya Y, Mikami M, Kawaura K, Endo M, Onishi K, Hayashi T, Sato K.
Title Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat.
Journal Cell Rep
Abstract Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.
Volume 28(5)
Pages 1362-1369.e4
Published 2019-7-30
DOI 10.1016/j.celrep.2019.06.090
PII S2211-1247(19)30870-8
PMID 31365876
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Gene Editing* Gene Knockout Techniques Genes, Recessive* Mutation* Plant Dormancy / genetics* Plant Proteins / genetics Plant Proteins / metabolism Seeds* / genetics Seeds* / metabolism Triticum* / genetics Triticum* / metabolism
IF 8.109
Times Cited 9
Barley BAC clone sequence LC054174 LC054175